Gene Expression Data
Assay Details
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Assay
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Reference:
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J:301026
Bogolyubova IO, et al., The dynamics of DAXX protein distribution in the nucleus of mouse early embryos. Acta Histochem. 2019 May;121(4):522-529
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7384958
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Gene symbol:
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Daxx
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Gene name:
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Fas death domain-associated protein
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Results
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Specimen
1 20h female pronucleus:
embryonic day 0.5
(more  )
Note: early zygote
Specimen
1 20h female pronucleus:
(close  )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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early zygote
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Specimen
1 20h male pronucleus:
embryonic day 0.5
(more )
Note: early zygote
Specimen
1 20h male pronucleus:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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early zygote
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Specimen
1 27h female pronucleus:
embryonic day 0.5
(more )
Note: late zygote
Specimen
1 27h female pronucleus:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based Age assigned by on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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late zygote
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
1_27h
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Noticeable areas of signal localization began to appear in association with the nucleolus precursor bodies (NPBs) in late zygotes. Immunopositive ring-shaped areas were observed around all the NPBs in female pronuclei.
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Specimen
late zygote (male pronucleus):
embryonic day 0.5
(more )
Note: late zygote
Specimen
late zygote (male pronucleus):
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based Age assigned by on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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late zygote
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
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Immunopositive ring-shaped areas were observed around the vast majority of NPBs in male pronuclei. However some male pronuclei (approx. in 25% zygotes) also contained NPBs demonstrating no such labeling.
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Specimen
1 46h:
embryonic day 1.0
(more )
Note: two-cell stage
Specimen
1 46h:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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two-cell stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
1_46h
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Labeling was observed in the heterochromatin rim at the periphery of nucleolus precursor bodies (NPBs) and in heterochromatin zones not associated with the NPBs.
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Specimen
1 72h:
embryonic day 3.0
(more )
Note: morula stage
Specimen
1 72h:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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morula stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Diffuse |
1_72h
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Diffuse labeling became prevalent again in morula stage embryos, with only a few foci of concentrated signal retained at the periphery of nucleoli.
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Specimen
4a:
embryonic day 0.5
(more )
Note: late zygote. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
Specimen
4a:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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late zygote. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
4a
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4a''
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4a'''
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Daxx accumulated in male and female pronuclei. There was no colocalization of Atrx with Daxx immunolabeling at the late zygote stage.
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Specimen
4b:
embryonic day 1.0
(more )
Note: two cell stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
Specimen
4b:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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two cell stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
4b
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4b'''
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4b''
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At late two-cell stage, colocalization of Daxx and ATRX was detectable both in the ring-shaped heterochromatin zones associated with the nucleolus precursor body (NPB) periphery and in NPB-unassociated heterochromatin patches. Some zones of Daxx localization did not overlap with ATRX (and vise versa). ATRX was found in 60% Daxx-positive heterochromatin areas associated with NPBs and in 80% Daxx-positive heterochromatin clumps located outside the NPBs.
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Specimen
4c:
embryonic day 3.0
(more )
Note: morula stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
Specimen
4c:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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morula stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Regionally restricted |
4c
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4c''
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4c'''
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There was no colocalization of Atrx with Daxx immunolabeling at the morula stage.
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Specimen
5a:
embryonic day 1.0
(more )
Note: two cell stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
Specimen
5a:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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two cell stage. Double labeled: red - Daxx; green - Atrx (assay MGI:7384973).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
5a''
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5a
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Daxx and Atrx colocalization in the nucleus of 2-cell mouse embryos.
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Analysis Tools