Pcna Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:314181 Holzman MA, et al., HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Front Cell Dev Biol. 2021;9:632303
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7384944
Gene symbol:	Pcna
Gene name:	proliferating cell nuclear antigen

Antibody:	PC10
Detection system:	Secondary antibody
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Anti PCNA (13110S) MGI:6388632 may have been used in this assay.

Results

S6A ©

S6B ©

Specimen S6A/B: embryonic day 11.5; Gt(ROSA)26Sor^{tm1.1(EYFP)Cos}/Gt(ROSA)26Sor⁺, Tg(Hoxa5-cre)447BLjea/0 (more

)
Note: Conditional mutant. Double labeled: magenta - Pcna; green - Cre reporter.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Widespread	S6B , S6A	Expression in virtually all somitic cells and derivatives.

S6C ©

S6D ©

Specimen S6C/D: embryonic day 12.5; Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Tg(Hoxa5-cre)447BLjea/0 (more

)
Note: Conditional mutant. Double labeled: green - Pcna; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Widespread	S6C , S6D	Expression in virtually all somitic cells and derivatives.

S6F ©

Specimen S6F: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: somite	Present	Widespread	S6F	Expression in virtually all somitic cells and derivatives.

S6G ©

Specimen S6G: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: somite	Present	Widespread	S6G	Expression in virtually all somitic cells and derivatives.

S6H ©

S6I ©

Specimen S6H/I: embryonic day 11.5; Gt(ROSA)26Sor^{tm1.1(EYFP)Cos}/Gt(ROSA)26Sor⁺, Hoxa5^tm1Rob/Hoxa5^tm1Rob, Tg(Hoxa5-cre)447BLjea/0 (more

)
Note: Conditional mutant. Double labeled: magenta - Pcna; green - Cre reporter.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Widespread	S6H , S6I	No obvious difference compared to control.

S6J ©

S6K ©

Specimen S6J/K: embryonic day 12.5; Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Hoxa5^tm1Rob/Hoxa5^tm1Rob, Tg(Hoxa5-cre)447BLjea/0 (more

)
Note: Conditional mutant. Double labeled: green - Pcna; red - Cre reporter.

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Widespread	S6K , S6J	No obvious difference compared to control.

S6M ©

Specimen S6M: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS22: somite	Present	Widespread	S6M	No obvious difference compared to control.

S6N ©

Specimen S6N: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS22: somite	Present	Widespread	S6N	No obvious difference compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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