Gene Expression Data
Assay Details
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Assay
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Reference:
|
J:260124
Goldmann T, et al., Origin, fate and dynamics of macrophages at central nervous system interfaces. Nat Immunol. 2016 Jul;17(7):797-805
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|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
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MGI:7382870
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|
Gene symbol:
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Cx3cr1
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|
Gene name:
|
C-X3-C motif chemokine receptor 1
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|
Reporter:
|
GFP
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|
Antibody:
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anti-GFP (600-106-215)
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|
Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
|
Specimen
1b:
postnatal; Cx3cr1tm1Litt/Cx3cr1 +
(more  )
Note: 3D reconstruction of confocal images. Triple labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869); red - Laminin.
Specimen
1b:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
3D reconstruction of confocal images. Triple labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869); red - Laminin.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
perivascular macrophage |
Present |
Regionally restricted |
1b
|
Expression was detected in perivascular macrophages, identified based on their close proximity to the abluminal side of Pecam1+ endothelial cells between the endothelial and the parenchymal basement membrane (laminin+), the perivascular space.
|
Specimen
1c:
postnatal; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: 3D reconstruction of confocal images. Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
1c:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
3D reconstruction of confocal images. Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain meninges |
meningeal macrophage |
Present |
Regionally restricted |
1c
|
Expression was detected in subdural meninges, localized by their close proximity to fibroblasts expressing ER-TR7 (fibroblast antibody).
|
Specimen
1h left:
postnatal adult; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
Specimen
1h left:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain meninges |
meningeal macrophage |
Present |
|
1h left
|
Expression was detected in meningeal macrophages co-expressing Ptprc.
|
| TS28: brain |
microglial cell |
Present |
|
1h left
|
Microglia expressed only Cx3cr1 reporter.
|
Specimen
1h center:
postnatal adult; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
Specimen
1h center:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
perivascular macrophage |
Present |
Regionally restricted |
1h center
|
Expression was detected in perivascular macrophages co-expressing Ptprc.
|
| TS28: brain |
microglial cell |
Present |
|
1h center
|
Microglia expressed only Cx3cr1 reporter.
|
Specimen
1h right:
postnatal adult; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
Specimen
1h right:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Ptprc (assay MGI:7382871).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: choroid plexus epithelium |
choroid plexus macrophage |
Present |
Regionally restricted |
1h right
|
Expression was detected in choroid plexus macrophages co-expressing Ptprc.
|
| TS28: choroid plexus stroma |
choroid plexus macrophage |
Present |
Regionally restricted |
1h right
|
Expression was detected in choroid plexus macrophages co-expressing Ptprc.
|
Specimen
1i:
postnatal adult; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Triple labeled: green - Cx3cr1; cyan - Mrc1 (assay MGI:7382872); red - Laminin.
Specimen
1i:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: green - Cx3cr1; cyan - Mrc1 (assay MGI:7382872); red - Laminin.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
perivascular macrophage |
Present |
Regionally restricted |
1i
|
Expression was detected in perivascular macrophages co-expressing Mrc1.
|
Specimen
2 Meninges E9.5:
embryonic day 9.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
2 Meninges E9.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: head mesenchyme |
meningeal macrophage |
Present |
Regionally restricted |
2_E9.5
|
Expression was detected in macrophages in the subdural meninges.
|
Specimen
2 Meninges E12.5:
embryonic day 12.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
2 Meninges E12.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: meninges |
meningeal macrophage |
Present |
|
2_E12.5 Meninges
|
|
Specimen
2 Meninges E13.5:
embryonic day 13.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
2 Meninges E13.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: meninges |
meningeal macrophage |
Present |
|
2_E13.5 Meninges
|
|
Specimen
2 Meninges E16.5:
embryonic day 16.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
2 Meninges E16.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: meninges |
meningeal macrophage |
Present |
|
2_E16.5 Meninges
|
|
Specimen
2 Meninges P60:
postnatal day 60; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
Specimen
2 Meninges P60:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - ER-TR7 fibroblasts.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: meninges |
meningeal macrophage |
Present |
|
2_P60 Meninges
|
|
Specimen
2 Perivascular space E12.5:
embryonic day 12.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
Specimen
2 Perivascular space E12.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: brain |
perivascular macrophage |
Present |
|
2_E12.5 Perivascular space
|
|
Specimen
2 Perivascular space E13.5:
embryonic day 13.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
Specimen
2 Perivascular space E13.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: brain |
perivascular macrophage |
Present |
|
2_E13.5 Perivascular space
|
|
Specimen
2 Perivascular space E16.5:
embryonic day 16.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
Specimen
2 Perivascular space E16.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: brain |
perivascular macrophage |
Present |
|
2_E16.5 Perivascular space
|
|
Specimen
2 Perivascular space P60:
postnatal day 60; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
Specimen
2 Perivascular space P60:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; cyan - Pecam1 (assay MGI:7382869).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
perivascular macrophage |
Present |
|
2_P60 Perivascular space
|
|
Specimen
2 Choroid plexus E12.5:
embryonic day 12.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Specimen
2 Choroid plexus E12.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2 Choroid plexus E13.5:
embryonic day 13.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Specimen
2 Choroid plexus E13.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2 Choroid plexus E16.5:
embryonic day 16.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Specimen
2 Choroid plexus E16.5:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2 Choroid plexus P60:
postnatal day 60; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Specimen
2 Choroid plexus P60:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3f Meninges Cx3cr1GFP/wt:
embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
Specimen
3f Meninges Cx3cr1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: meninges |
meningeal macrophage |
Present |
|
3f Meninges Cx3cr1GFP/WT
|
GFP and Aif1 expression co-localized in meningeal macrophages.
|
Specimen
3f Choroid plexus Cx3cr1GFP/wt:
embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
Specimen
3f Choroid plexus Cx3cr1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: choroid plexus epithelium |
choroid plexus macrophage |
Present |
|
3f Choriod plexus Cx3cr1GFP/WT
|
GFP and Aif1 expression co-localized in choroid plexus macrophages.
|
| TS22: choroid plexus stroma |
choroid plexus macrophage |
Present |
|
3f Choriod plexus Cx3cr1GFP/WT
|
GFP and Aif1 expression co-localized in choroid plexus macrophages.
|
Specimen
3f Meninges Irf8<-/->;Cx3cr1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
Irf8tm1Hor/Irf8tm1Hor
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: meninges |
meningeal macrophage |
Present |
|
3f Meninges Irf8-/- Cx3cr1GFP/WT
|
There was a reduction in the number of meningeal macrophages noted in the Irf8 mutant background.
|
Specimen
3f Choroid plexus Irf8<-/->;Cx3cr1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Cx3cr1tm1Litt/Cx3cr1+
Irf8tm1Hor/Irf8tm1Hor
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Cx3cr1; red - Aif1 (assay MGI:7382874).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: choroid plexus epithelium |
choroid plexus macrophage |
Present |
|
3f Choriod plexus Irf8-/- Cx3cr1GFP/WT
|
Choroid plexus macrophages were not affected in the Irf8 mutant background.
|
| TS22: choroid plexus stroma |
choroid plexus macrophage |
Present |
|
3f Choriod plexus Irf8-/- Cx3cr1GFP/WT
|
Choroid plexus macrophages were not affected in the Irf8 mutant background.
|
|
Analysis Tools