Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:158583
Ko HW, et al., Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction. Dev Cell. 2010 Feb 16;18(2):237-47
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:7380680
|
|
Gene symbol:
|
Ptch1
|
|
Gene name:
|
patched 1
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1B +/+,Ptch/+:
embryonic day 10.5; Ptch1tm1Mps/Ptch1 +
(more  )
Specimen
1B +/+,Ptch/+:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ptch1tm1Mps/Ptch1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Graded |
1B +/+,Ptch1/+
|
Expression in a ventral-to-dorsal gradient.
|
Specimen
1B bromi,Ptch/+:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Ptch1tm1Mps/Ptch1+
Tbc1d32bromi/Tbc1d32bromi
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Weak |
Regionally restricted |
1B bromi,Ptch1/+
|
Expression in ventral one-third consistently weaker compared to wild type.
|
Specimen
S2B bromi,Ptch/+:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Ptch1tm1Mps/Ptch1+
Tbc1d32bromi/Tbc1d32bromi
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
|
S2B bromi,Ptch1/+
|
|
Specimen
S2B Ptch1:
embryonic day 9.5; Ptch1tm1Mps/Ptch1 +
(more )
Specimen
S2B Ptch1:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ptch1tm1Mps/Ptch1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Strong |
|
S2B Ptch1
|
|
Specimen
S2B bromi,Ptch1:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Widespread |
S2B bromi,Ptch1
|
Expression more widespread compared to bromi,Ptch1/+ embryos, but considerably weaker than Ptch1 single mutants.
|
|
Analysis Tools