Gene Expression Data
Assay Details
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Assay
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Reference:
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J:158583
Ko HW, et al., Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction. Dev Cell. 2010 Feb 16;18(2):237-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7380668
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Antibody:
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74.5A5
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Detection system:
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Secondary antibody
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Results
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Specimen
1A Nkx2.2 WT:
embryonic day 10.5
(more  )
Specimen
1A Nkx2.2 WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
1A Nkx2.2 WT
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Specimen
1A Nkx2.2 bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Weak |
Regionally restricted |
1A Nkx2.2 bromi
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Reduced number of expressing reduced and inappropriately positioned in ventral midline.
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Specimen
2 Nkx2.2 WT:
embryonic day 9.5
(more )
Specimen
2 Nkx2.2 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2 Nkx2.2 bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2 Nkx2.2 Ptch1:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1tm1Mps
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Widespread |
2_Nkx2.2 Ptch1
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Broadly expressed throughout open neural tube.
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Specimen
2 Nkx2.2 bromi,Ptch1:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Absent |
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2_Nkx2.2 bromi,Ptch1
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Expression pattern similar to bromi single mutant.
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Specimen
3A Nkx2.2 WT:
embryonic day 10.5
(more )
Specimen
3A Nkx2.2 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3A Nkx2.2 bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3A Nkx2.2 Gli3xt:
(close )
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Genetic Background:
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involves: C3H/HeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
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3A Nkx2.2 Gli3xt
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Authors report normal expression.
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Specimen
3A Nkx2.2 bromi,Gli3xt:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3A Nix2.2 bromi,Gli3xt
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Authors report disruption of floor plate specification.
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Specimen
3B Nkx2.2 WT:
embryonic day 10.5
(more )
Specimen
3B Nkx2.2 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3B Nkx2.2 bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3B Nkx2.2 Gli2:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Weak |
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3B Nkx2.2 Gli2
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Reduced numbers of expressing cells.
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Specimen
3B Nkx2.2 bromi,Gli2:
(close )
Specimen
S2C Nkx2.2 WT:
embryonic day 10.5
(more )
Specimen
S2C Nkx2.2 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
S2C Nkx2.2 bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
S2C Nkx2.2 Rab23opb2:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Rab23opb2/Rab23opb2
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
S2C Nkx2.2 Rab23opb2
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Expression domain shifted compared to wild type.
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Specimen
S2C Nkx2.2 bromi,Rab23opb2:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Weak |
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S2C Nkx2.2 bromi,Rab23opb2
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Authors report same dorsalized phenotype as in bromi single mutants.
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Analysis Tools