Nkx2-2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:158583 Ko HW, et al., Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction. Dev Cell. 2010 Feb 16;18(2):237-47
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7380668
Gene symbol:	Nkx2-2
Gene name:	NK2 homeobox 2

Antibody:	74.5A5
Detection system:	Secondary antibody

Results

Specimen 1A Nkx2.2 WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1A Nkx2.2 WT

Specimen 1A Nkx2.2 bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak	Regionally restricted	1A Nkx2.2 bromi	Reduced number of expressing reduced and inappropriately positioned in ventral midline.

Specimen 2 Nkx2.2 WT: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2_Nkx2.2 WT

Specimen 2 Nkx2.2 bromi: embryonic day 9.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		2_Nkx2.2 bromi

Specimen 2 Nkx2.2 Ptch1: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Widespread	2_Nkx2.2 Ptch1	Broadly expressed throughout open neural tube.

Specimen 2 Nkx2.2 bromi,Ptch1: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		2_Nkx2.2 bromi,Ptch1	Expression pattern similar to bromi single mutant.

Specimen 3A Nkx2.2 WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3A Nkx2.2 WT

Specimen 3A Nkx2.2 bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak		3A Nkx2.2 bromi

Specimen 3A Nkx2.2 Gli3xt: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3A Nkx2.2 Gli3xt	Authors report normal expression.

Specimen 3A Nkx2.2 bromi,Gli3xt: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3A Nix2.2 bromi,Gli3xt	Authors report disruption of floor plate specification.

Specimen 3B Nkx2.2 WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3B Nkx2.2 WT

Specimen 3B Nkx2.2 bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak		3B Nkx2.2 bromi

Specimen 3B Nkx2.2 Gli2: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak		3B Nkx2.2 Gli2	Reduced numbers of expressing cells.

Specimen 3B Nkx2.2 bromi,Gli2: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		3B Nkx2.2 bromi,Gli2

Specimen S2C Nkx2.2 WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		S2C Nkx2.2 WT

Specimen S2C Nkx2.2 bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak		S2C Nkx2.2 bromi

Specimen S2C Nkx2.2 Rab23opb2: embryonic day 10.5; Rab23^opb2/Rab23^opb2 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	S2C Nkx2.2 Rab23opb2	Expression domain shifted compared to wild type.

Specimen S2C Nkx2.2 bromi,Rab23opb2: embryonic day 10.5; Rab23^opb2/Rab23^opb2, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak		S2C Nkx2.2 bromi,Rab23opb2	Authors report same dorsalized phenotype as in bromi single mutants.

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