Gene Expression Data
Assay Details
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Assay
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Reference:
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J:158583
Ko HW, et al., Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction. Dev Cell. 2010 Feb 16;18(2):237-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7380665
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Antibody:
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5E1
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Detection system:
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Secondary antibody
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Results
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Specimen
1A Shh WT:
embryonic day 10.5
(more  )
Specimen
1A Shh WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
1A Shh bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2 Shh WT:
embryonic day 9.5
(more )
Specimen
2 Shh WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2 Shh bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2 Shh Ptch1:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1tm1Mps
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Widespread |
2_Shh Ptch1
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Broadly expressed throughout open neural tube.
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Specimen
2 Shh bromi,Ptch1:
(close )
Specimen
S2C Shh WT:
embryonic day 10.5
(more )
Specimen
S2C Shh WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
S2C Shh bromi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tbc1d32bromi/Tbc1d32bromi
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
S2C Shh Rab23opb2:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Rab23opb2/Rab23opb2
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
S2C Shh Rab23opb2
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Expression domain shifted compared to wild type.
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Specimen
S2C Shh bromi,Rab23opb2:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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S2C Shh bromi,Rab23opb2
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Authors report same dorsalized phenotype as in bromi single mutants.
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Specimen
S4B Shh WT:
embryonic day 10.5
(more )
Specimen
S4B Shh WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
S4B Shh RRF/RRF:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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S4B Shh RRF/RRF
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Expression identical to that of bromi mutants.
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| TS17: notochord |
Present |
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S4B Shh RRF/RRF
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Analysis Tools