Shh Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:158583 Ko HW, et al., Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction. Dev Cell. 2010 Feb 16;18(2):237-47
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7380665
Gene symbol:	Shh
Gene name:	sonic hedgehog

Antibody:	5E1
Detection system:	Secondary antibody

Results

Specimen 1A Shh WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		1A Shh WT
TS17: notochord	Present		1A Shh WT

Specimen 1A Shh bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		1A Shh bromi
TS17: notochord	Present		1A Shh bromi

Specimen 2 Shh WT: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		2_Shh WT

Specimen 2 Shh bromi: embryonic day 9.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		2_Shh bromi

Specimen 2 Shh Ptch1: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Widespread	2_Shh Ptch1	Broadly expressed throughout open neural tube.

Specimen 2 Shh bromi,Ptch1: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		2_Shh bromi,Ptch1	Expression pattern similar to bromi single mutant.

Specimen S2C Shh WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S2C Shh WT
TS17: notochord	Present		S2C Shh WT

Specimen S2C Shh bromi: embryonic day 10.5; Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		S2C Shh bromi
TS17: notochord	Present		S2C Shh bromi

Specimen S2C Shh Rab23opb2: embryonic day 10.5; Rab23^opb2/Rab23^opb2 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	S2C Shh Rab23opb2	Expression domain shifted compared to wild type.

Specimen S2C Shh bromi,Rab23opb2: embryonic day 10.5; Rab23^opb2/Rab23^opb2, Tbc1d32^bromi/Tbc1d32^bromi (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		S2C Shh bromi,Rab23opb2	Authors report same dorsalized phenotype as in bromi single mutants.

Specimen S4B Shh WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S4B Shh WT
TS17: notochord	Present		S4B Shh WT

Specimen S4B Shh RRF/RRF: embryonic day 10.5; Tbc1d32^{Gt(RRF165)Byg}/Tbc1d32^{Gt(RRF165)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		S4B Shh RRF/RRF	Expression identical to that of bromi mutants.
TS17: notochord	Present		S4B Shh RRF/RRF

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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