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Gene Expression Data
Assay Details
Assay
Reference: J:308699 Bjorke B, et al., Oculomotor nerve guidance and terminal branching requires interactions with differentiating extraocular muscles. Dev Biol. 2021 Aug;476:272-281
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7378839
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Antibody: Anti-Pitx2ABC (PA 1020-100)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2A: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present 2A
TS20: face Present Regionally restricted 2A Expressed in orbital tissues.
Specimen 2C: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: skeletal musculature Present Regionally restricted 2C Expressed in the medial rectus and other typical muscles, with no nerve fibers yet inserted.
Specimen 2D: embryonic day 13.0 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS21: extrinsic ocular pre-muscle mass Present 2D Expressed in the superior and inferior rectus extraocular muscles, but generally were not penetrated by nerves, except the superior rectus by the superior branch. Myofibers established their stereotypical coordinate positions around the eye, extending from the eye to the orbit.
Specimen 2E: embryonic day 13.0 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS21: extrinsic ocular pre-muscle mass Present 2E Expressed in the superior and lateral rectus extraocular muscles, but generally were not penetrated by nerves, except the superior rectus by the superior branch. Myofibers established their stereotypical coordinate positions around the eye, extending from the eye to the orbit.
Specimen 2F: embryonic day 13.0 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS21: extrinsic ocular pre-muscle mass Present Regionally restricted 2F Expressed in the superior, lateral, medial and inferior rectus extraocular muscles, but generally were not penetrated by nerves, except the superior rectus by the superior branch.
Specimen 4A: embryonic day 10.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS17: eye Present Regionally restricted 4A Expressed in the future extraocular muscles.
Specimen 4B: embryonic day 10.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS17: eye Present Regionally restricted 4B Expressed in the future extraocular muscles. Fewer and less condensed expression than in controls, spreading over a larger area.
Specimen 4C: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present 4C Expressed in the superior, lateral and inferior rectus, superior oblique extraocular muscles. Additional Pitx2+ cells formed layers around the optic nerve and cup.
Specimen 4D: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Pitx2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present 4D The Pitx2+ masses in the expected muscle positions were much smaller, and less densely packed.
TS20: eye mesenchyme Present Regionally restricted 4D Pitx2+ layers remained around the optic nerve and cup (non-muscle tissues).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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