Cd3e Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7343679
Gene symbol:	Cd3e
Gene name:	CD3 antigen, epsilon polypeptide

Antibody:	anti-CD3 (E4T1B; 78588)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).

Results

S3b CD3 ©

S3b Merge ©

Specimen S3b: postnatal day 120; Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}/Gt(ROSA)26Sor⁺, Ihh^{em1(Tomm20/mKate2,dre)Xya}/Ihh⁺ (more

)
Note: Conditional mutant. Multi-labeled: green - Cd3e; red - Ihh reporter (assay MGI:7341718); magenta - Cd207 (assay MGI:7343677); yellow - S100a1 (assay MGI:7343682); cyan - Dre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: skin	Langerhans cell	Present		S3b Merge inset , S3b CD3 , S3b Merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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