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Gene Expression Data
Assay Details
Assay
Reference: J:216076 Hou C, et al., Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice. Dev Biol. 2014 Nov 1;395(1):167-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7341515
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 10A: postnatal day 3 (more )
Note: After 30 minutes pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present Regionally restricted 10A Expressed in the outer layer.
Specimen 10B: postnatal day 3; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 30 minutes pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 10B The proportion of Mki67 positive cells labelled by BrdU was significantly lower than wild type.
Specimen 10D: postnatal day 3 (more )
Note: After 24 hours pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 10D
Specimen 10E: postnatal day 3; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 24 hours pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present 10E Similar differentiation rate in mutant compared to wild-type mice.
Specimen S2A: embryonic day 13.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. Double labeled: red - Mki67; green - Tubb3 (assay MGI:7341507).
Structure Level Pattern Image Note
TS21: cerebellum Present Regionally restricted S2A Most of the positive cells were located at the cortex.
Specimen S3E: postnatal day 2 (more )
Note: After 30 minutes pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present Regionally restricted S3E Expressed in the outer layer.
Specimen S3F: postnatal day 2; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 30 minutes pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present Regionally restricted S3F Expressed in the outer layer, similar as in the wild-type.
Specimen S3H: postnatal day 3 (more )
Note: After 24 hours pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present Regionally restricted S3H Expressed in the outer layer.
Specimen S3I: postnatal day 3; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 24 hours pulse labeling of BrdU. Double labeled: red - Mki67; green - anti-BrdU antibody.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present Regionally restricted S3I Expressed in the outer layer, similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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