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Gene Expression Data
Assay Details
Assay
Reference: J:216076 Hou C, et al., Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice. Dev Biol. 2014 Nov 1;395(1):167-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7341507
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tuj1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5Ca/c: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: cerebellum neuron Present Regionally restricted 5Cc , 5Ca Expressed throughout the cerebellar primordium except for the apical surface of ventricular zone.
Specimen 5Cd/f: embryonic day 13.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: cerebellum neuron Present Regionally restricted 5Cf , 5Cd Significantly weaker expression compared to wild-type (quantified in Fig 5E). Some positive cells were misplaced at the apical surface of the ventricular zone.
TS21: cerebellum ventricular layer neuron Present Regionally restricted 5Cf , 5Cd Some positive cells were misplaced at the apical surface of the ventricular zone.
Specimen S2A: embryonic day 13.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. Double labeled: green - Tubb3; red - Mki67 (assay MGI:7341515).
Structure Cell Type Level Pattern Image Note
TS21: cerebellum ventricular layer neuron Present Regionally restricted S2A Some positive cells were misplaced at the apical surface of the ventricular zone. Most of the Mki67-positive cells were located at the cortex.
Specimen S2B: embryonic day 13.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. Double labeled: green - Tubb3; red - Pax6 (assay MGI:7341501).
Structure Cell Type Level Pattern Image Note
TS21: cerebellum layer cerebellar granule cell Present Regionally restricted S2B Some cells coexpressed with Pax6 in the external granule cell layer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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