Antibody:	anti-Hugl-1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS22: cerebellum layer	Strong	Regionally restricted	1A	Expressed throughout the cerebellum and was highly expressed in the external granule cell layer.
TS22: cerebellum ventricular layer	Strong	Regionally restricted	1A	Highly expressed in the ventral ventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Weak		1B
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Weak		1B
TS28: cerebellum Purkinje cell layer		Strong		1B
TS28: cerebellum molecular layer		Strong		1B
TS28: cerebellum white matter		Strong		1B	Expressed in the white matter fibre tracts.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer		Strong		1C	Expression remained high in the dendrites.
TS28: cerebellum white matter		Absent		1C
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present	Scattered	1C

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebellum layer	cerebellar granule cell	Strong	Regionally restricted	1D	Coexpressed with Pax6 in granule cells in the external granule cell layer.

Structure	Level	Pattern	Image	Note
TS22: deep cerebellar nucleus	Present		1E	Coexpressed with Tbr1 in the transitory zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Strong		1F
TS28: cerebellum white matter		Strong		1F	Expressed in white matter glia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		1G	Coexpressed with Calb1 in Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter		Strong		2A
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		2A	Highly expressed in Purkinje cell dendrites.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		2B