Gfap Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:216076 Hou C, et al., Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice. Dev Biol. 2014 Nov 1;395(1):167-81
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7341503
Gene symbol:	Gfap
Gene name:	glial fibrillary acidic protein

Antibody:	anti-GFAP
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

1F ©

Specimen 1F: postnatal day 10 (more

)
Note: Double labeled: red - Gfap; green - Llgl1 (assay MGI:7341499).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	glial cell	Present		1F

9A ©

Specimen 9A: postnatal day 11 (more

)
Note: Double labeled: red - Gfap; green - Rbfox3 (assay MGI:7341516).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Bergmann glial cell	Strong		9A	Positive cells arranged in the molecular layer in a tight and orderly manner.

9B ©

Specimen 9B: postnatal day 11; Llgl1^tm1Vv/Llgl1^tm1Vv, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - Rbfox3 (assay MGI:7341516).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Bergmann glial cell	Present		9B	Shortened Bergmann glia fibres were loosely placed in the molecular layer.

9C ©

Specimen 9C: postnatal day 11 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter	glial cell	Strong		9C

9D ©

Specimen 9D: postnatal day 11; Llgl1^tm1Vv/Llgl1^tm1Vv, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter	glial cell	Present		9D	Weaker expression compared with the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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