Gene Expression Data
Assay Details
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Assay
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Reference:
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J:237675
Wyler SC, et al., Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons. ACS Chem Neurosci. 2015 Jul 15;6(7):1198-205
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7341495
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Gene symbol:
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Slc22a3
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Gene name:
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solute carrier family 22 (organic cation transporter), member 3
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Probe:
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Slc22a3 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4B WT:
postnatal adult
(more  )
Specimen
4B WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
Present |
Regionally restricted |
4B WT
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A highly restricted serotonergic raphe pattern of expression was noted.
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| TS28: pons |
Present |
Regionally restricted |
4B WT
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A highly restricted serotonergic raphe pattern of expression was noted.
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Specimen
4B Pet-1-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fevtm1Esd/Fevtm1Esd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
5A:
postnatal adult
(more )
Note: Fig. 5 showed sections through the entire dorsal raphe in an anterior to posterior series. 5A - Anterior-most section of wild type dorsal raphe.
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Fig. 5 showed sections through the entire dorsal raphe in an anterior to posterior series. 5A - Anterior-most section of wild type dorsal raphe.
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Specimen
5B:
postnatal adult
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
postnatal adult
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
postnatal adult
(more )
Note: 5D - Posterior-most section of wild type dorsal raphe.
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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5D - Posterior-most section of wild type dorsal raphe.
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Specimen
5E:
postnatal adult; Fevtm1Esd/ Fevtm1Esd
(more )
Note: 5E - Anterior-most section of Pet-1-/- dorsal raphe.
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fevtm1Esd/Fevtm1Esd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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5E - Anterior-most section of Pet-1-/- dorsal raphe.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
Trace |
Scattered |
5E
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A nearly complete elimination of expression was noted at all levels of the dorsal raphe in the mutant mice. Residual expression, if any, was confined to scattered cells.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fevtm1Esd/Fevtm1Esd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
Trace |
Scattered |
5F
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A nearly complete elimination of expression was noted at all levels of the dorsal raphe in the mutant mice. Residual expression, if any, was confined to scattered cells.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fevtm1Esd/Fevtm1Esd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
Trace |
Scattered |
5G
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A nearly complete elimination of expression was noted at all levels of the dorsal raphe in the mutant mice. Residual expression, if any, was confined to scattered cells.
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Specimen
5H:
postnatal adult; Fevtm1Esd/ Fevtm1Esd
(more )
Note: 5H - Posterior-most section of Pet-1-/- dorsal raphe.
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fevtm1Esd/Fevtm1Esd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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5H - Posterior-most section of Pet-1-/- dorsal raphe.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
Trace |
Scattered |
5H
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A nearly complete elimination of expression was noted at all levels of the dorsal raphe in the mutant mice. Residual expression, if any, was confined to scattered cells.
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