Oxtr In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:324880 Inoue YU, et al., Targeting Neurons with Functional Oxytocin Receptors: A Novel Set of Simple Knock-In Mouse Lines for Oxytocin Receptor Visualization and Manipulation. eNeuro. 2022 Jan-Feb;9(1):ENEURO.0423-21.2022
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7336800
Gene symbol:	Oxtr
Gene name:	oxytocin receptor

Reporter:	other (see notes)
Antibody:	anti-PA tag (clone NZ-1) (016-25861)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Podoplanin, included in the allele construct, was used as the reporter in this assay.

Results

2e ©

Specimen 2e: postnatal week 2; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		2e
TS28: cerebral cortex	Present		2e
TS28: dentate gyrus	Present		2e
TS28: hippocampus CA2	Present	Regionally restricted	2e	Expression is seen in the anterior.
TS28: hippocampus CA3	Present	Regionally restricted	2e	Expression is seen in the anterior.
TS28: paraventricular thalamic nucleus	Present		2e
TS28: central amygdaloid nucleus	Present		2e
TS28: endopiriform nucleus	Present	Regionally restricted	2e	Expression is seen dorsally.
TS28: posteromedial cortical amygdaloid nucleus	Present		2e

2h ©

Specimen 2h: postnatal week 2; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Double labeled: green - Oxtr PA reporter; red - Oxtr tdTomato reporter (assay MGI:7336798).

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		2h
TS28: cortical layer IV	Present		2h

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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