Oxtr In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:324880 Inoue YU, et al., Targeting Neurons with Functional Oxytocin Receptors: A Novel Set of Simple Knock-In Mouse Lines for Oxytocin Receptor Visualization and Manipulation. eNeuro. 2022 Jan-Feb;9(1):ENEURO.0423-21.2022
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7336798
Gene symbol:	Oxtr
Gene name:	oxytocin receptor

Reporter:	tdTomato
Antibody:	anti-tdTomato-GP-Af430
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2d ©

Specimen 2d: postnatal week 2; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		2d
TS28: cerebral cortex	Present		2d
TS28: dentate gyrus	Present		2d
TS28: hippocampus CA2	Present	Regionally restricted	2d	Expression is seen in the anterior.
TS28: hippocampus CA3	Present	Regionally restricted	2d	Expression is seen in the anterior.
TS28: paraventricular thalamic nucleus	Present		2d
TS28: central amygdaloid nucleus	Present		2d
TS28: endopiriform nucleus	Present	Regionally restricted	2d	Expression is seen dorsally.
TS28: posteromedial cortical amygdaloid nucleus	Present		2d

2h ©

Specimen 2h: postnatal week 2; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Double labeled: red - Oxtr tdTomato reporter; green - Oxtr PA reporter (assay MGI:7336800).

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		2h
TS28: cortical layer IV	Present		2h

4l ©

4m ©

4n ©

4o ©

4p ©

Specimen 4l: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		4l , 4m
TS28: cortical layer IV	Present		4l , 4m
TS28: dentate gyrus	Present		4l , 4n
TS28: hippocampus CA2	Present	Regionally restricted	4l , 4n	Expression is seen in the anterior.
TS28: hippocampus CA3	Present	Regionally restricted	4l , 4n	Expression is seen in the anterior.
TS28: paraventricular thalamic nucleus	Present		4o , 4l
TS28: endopiriform nucleus	Present	Regionally restricted	4p , 4l	Expression is seen dorsally.
TS28: central amygdaloid nucleus	Present		4p , 4l

Extended Data 3-1c ©

Extended Data 3-1d ©

Specimen Ext Data 3-1c,d: postnatal week 5; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma 1.94 mm

Structure	Cell Type	Level	Image
TS28: prelimbic area		Present	Extended Data 3-1c
TS28: cortical layer II	cortical interneuron	Present	Extended Data 3-1c , Extended Data 3-1d
TS28: cortical layer V	cortical interneuron	Present	Extended Data 3-1d , Extended Data 3-1c

Extended Data 3-1f ©

Specimen Ext Data 3-1f: postnatal week 5; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Double labeled: red - Oxtr reporter; blue - Cux1 (assay MGI:7336803).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	glutamatergic neuron	Present		Extended Data 3-1f

Extended Data 3-1h ©

Specimen Ext Data 3-1h: postnatal week 5; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Double labeled: red - Oxtr reporter; green - Sst (assay MGI:7336804).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer V	GABAergic interneuron	Present		Extended Data 3-1h

Extended Data 4-1b ©

Specimen Ext Data 4-1b: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma 1.78 mm

Structure	Level	Pattern	Image	Note
TS28: primary motor cortex	Present		Extended Data 4-1b
TS28: primary somatosensory cortex	Present		Extended Data 4-1b
TS28: endopiriform nucleus	Present	Regionally restricted	Extended Data 4-1b	Expression is seen in the dorsal and intermediate endopiriform claustrum.
TS28: prelimbic area	Present		Extended Data 4-1b

Extended Data 4-1d ©

Specimen Ext Data 4-1d: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma 1.42 mm

Structure	Level	Image	Note
TS28: primary somatosensory cortex	Present	Extended Data 4-1d
TS28: accumbens nucleus	Present	Extended Data 4-1d	Expression is seen in the core and shell.
TS28: cingulate cortex	Present	Extended Data 4-1d

Extended Data 4-1f ©

Specimen Ext Data 4-1f: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma 0.62 mm

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present		Extended Data 4-1f

Extended Data 4-1h ©

Specimen Ext Data 4-1h: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma -0.10 mm

Structure	Level	Pattern	Image	Note
TS28: lateral septal complex	Present		Extended Data 4-1h

Extended Data 4-1j ©

Specimen Ext Data 4-1j: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma -0.58 mm

Structure	Level	Pattern	Image	Note
TS28: endopiriform nucleus	Present	Regionally restricted	Extended Data 4-1j	Expression is seen dorsally.

Extended Data 4-1l ©

Specimen Ext Data 4-1l: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma -2.30 mm

Structure	Level	Pattern	Image	Note
TS28: primary visual cortex	Present		Extended Data 4-1l
TS28: secondary visual cortex	Present	Regionally restricted	Extended Data 4-1l	Expression is seen in the medial and lateral areas.
TS28: auditory cortex	Present		Extended Data 4-1l
TS28: posteromedial cortical amygdaloid nucleus	Present		Extended Data 4-1l

Extended Data 4-1n ©

Specimen Ext Data 4-1n: postnatal week 11; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma -2.92 mm

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present		Extended Data 4-1n

Specimen Ext Data 5-1c - Sample 1: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

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Note: Bregma 0.62 mm

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Present		Extended Data 5-1c Normal diet: 1

Specimen Ext Data 5-1c - Sample 2: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma 0.62 mm

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Present		Extended Data 5-1c Normal diet: 2

Specimen Ext Data 5-1c - Sample 3: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma 0.62 mm

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Present		Extended Data 5-1c Normal diet: 3

Specimen Ext Data 5-1d - Sample 1: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: ventromedial hypothalamic nucleus	Present		Extended Data 5-1d Normal diet: 1
TS28: medial amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 1
TS28: posteromedial cortical amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 1
TS28: endopiriform nucleus	Present	Regionally restricted	Extended Data 5-1d Normal diet: 1	Expression is seen dorsally.

Specimen Ext Data 5-1d - Sample 2: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: ventromedial hypothalamic nucleus	Present		Extended Data 5-1d Normal diet: 2
TS28: medial amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 2
TS28: posteromedial cortical amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 2
TS28: endopiriform nucleus	Present	Regionally restricted	Extended Data 5-1d Normal diet: 2	Expression is seen dorsally.

Specimen Ext Data 5-1d - Sample 3: postnatal week 6; Oxtr^em1Yinn/Oxtr^em1Yinn (more

)
Note: Bregma -1.82 mm

Structure	Level	Pattern	Image	Note
TS28: ventromedial hypothalamic nucleus	Present		Extended Data 5-1d Normal diet: 3
TS28: medial amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 3
TS28: posteromedial cortical amygdaloid nucleus	Present		Extended Data 5-1d Normal diet: 3
TS28: endopiriform nucleus	Present	Regionally restricted	Extended Data 5-1d Normal diet: 3	Expression is seen dorsally.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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