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Gene Expression Data
Assay Details
Assay
Reference: J:274541 Ito K, et al., Gene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice. J Biol Chem. 2014 Oct 24;289(43):29892-911
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7335127
Gene symbol: Bex2
Gene name: brain expressed X-linked 2
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5B EGFP/Y: embryonic day 13.5; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS21: embryo Present 5B EGFP/Y Expression was higher than the female littermates.
Specimen 5B EGFP/X: embryonic day 13.5; Bex2tm1Kami/Bex2+ (more )
Structure Level Pattern Image Note
TS21: embryo Present 5B EGFP/X
Specimen 5C EGFP/Y intestine: embryonic day 13.5; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS21: intestine Present 5C intestine EGFP/Y Greater number of positive cells compared with the female controls.
Specimen 5C EGFP/X intestine: embryonic day 13.5; Bex2tm1Kami/Bex2+ (more )
Structure Level Pattern Image Note
TS21: intestine Present 5C intestine EGFP/X
Specimen 5C EGFP/Y kidney: embryonic day 13.5; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS21: metanephros Present 5C kidney EGFP/Y Greater number of positive cells compared with the female controls.
Specimen 5C EGFP/X kidney: embryonic day 13.5; Bex2tm1Kami/Bex2+ (more )
Structure Level Pattern Image Note
TS21: metanephros Present 5C kidney EGFP/X
Specimen 5C EGFP/Y testis: embryonic day 13.5; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS21: testis Present 5C testis
Specimen 5C EGFP/X ovary: embryonic day 13.5; Bex2tm1Kami/Bex2+ (more )
Structure Level Pattern Image Note
TS21: ovary Present 5C ovary
Specimen 6A EGFP/Y: embryonic day 13.5; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS21: liver Strong Clusters 6A EGFP/Y Greater number of positive cells compared with the female controls.
Specimen 6A EGFP/X: embryonic day 13.5; Bex2tm1Kami/Bex2+ (more )
Structure Level Pattern Image Note
TS21: liver Strong Clusters 6A EGFP/X
Specimen 10A Retina: postnatal week 9; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS28: neural retina Present Widespread 10A right
Specimen 10B left: postnatal week 9; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS28: brain Present Widespread 10B left
Specimen 10B middle: postnatal week 9; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS28: brain Present Widespread 10B middle
Specimen 10B right: postnatal week 9; Bex2tm1Kami/Y (more )
Structure Level Pattern Image Note
TS28: brain Present Widespread 10B right
Specimen 11B upper: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Ins1 (assay MGI:7335160) and Ins2 (assay MGI:7335178).
Structure Cell Type Level Pattern Image Note
TS28: endocrine pancreas type B pancreatic cell Present 11B upper Coexpressed with insulin.
Specimen 11B middle: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Sst (assay MGI:7335161).
Structure Cell Type Level Pattern Image Note
TS28: endocrine pancreas pancreatic A cell Present 11B middle Coexpressed with Sst.
Specimen 11B lower: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Gcg (assay MGI:7335162).
Structure Cell Type Level Pattern Image Note
TS28: endocrine pancreas pancreatic PP cell Present 11B lower Coexpressed with Gcg.
Specimen 11C: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Chga (assay MGI:7335165).
Structure Cell Type Level Pattern Image Note
TS28: small intestine epithelium enteroendocrine cell of small intestine Present Regionally restricted 11C merge , 11C EGFP Intestinal epithelial EGFP-expressing cells coexpressed Chga. Positive cells near the muscular layer do not express Chga.
TS28: small intestine mesentery Strong Regionally restricted 11C merge , 11C EGFP , 11Cb Expressed at the submucosal region, a structure representing the neural lineage.
Specimen 11D left: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Sox9 (assay MGI:7335166).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 11D left Coexpressed with Sox9.
Specimen 11D right: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Wt1 (assay MGI:7335167).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 11D right Coexpressed with Wt1.
Specimen 11E left: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Zbtb16 (assay MGI:7335168).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 11E left Not colocalized with Zbtb16/Plzf.
Specimen 11E middle: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Gcna (assay MGI:7335169).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 11E middle Not colocalized with Gcna/Tra98.
Specimen 11E right: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Ddx4 (assay MGI:7335170).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 11E right Not colocalized with Ddx4/Mvh.
Specimen 12A forestomach: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Chga (assay MGI:7335165).
Structure Level Pattern Image Note
TS28: stomach proventricular region Absent 12A upper EGFP , 12A upper merge
Specimen 12A corpus: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Chga (assay MGI:7335165).
Structure Level Pattern Image Note
TS28: body of stomach Present Single cells 12A middle EGFP , 12A middle merge Very rare in the corpus region.
Specimen 12A pyloric stomach: postnatal week 9; Bex2tm1Kami/Y (more )
Note: Double labeled: green - Bex2 reporter; red - Chga (assay MGI:7335165).
Structure Cell Type Level Pattern Image Note
TS28: stomach pyloric region stomach enteroendocrine cell Present 12A lower EGFP , 12A lower merge Enriched at the bases of glands. A subset of positive cells were Chga+ endocrine cells. Many Chga-EGFP+ cells were detected.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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