Hoxc13 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:297683 Fernandez-Guerrero M, et al., Mammalian-specific ectodermal enhancers control the expression of Hoxc genes in developing nails and hair follicles. Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30509-30519
Assay type:	RT-PCR
MGI Accession ID:	MGI:7335083
Gene symbol:	Hoxc13
Gene name:	homeobox C13

Probe:	Hoxc13-pK, Hoxc13-pL
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh. The level of expression for wild type control was set to 1.

Results

Image: 6 ©

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Wt	E15.5	TS23: forelimb digit	Present	0.5 µg; total RNA	Not Specified		Not Specified	Sample was forelimb digit tips.
Wt	E15.5	TS23: forelimb digit	Present	0.5 µg; total RNA	Not Specified		Not Specified	Sample was forelimb digit tips.
HoxCKO Het	E15.5	TS23: forelimb digit	Present	0.5 µg; total RNA	involves: 129S/SvEv * C57BL/6	Hoxc^tm1Nogu/Hoxc⁺	Not Specified	Sample was forelimb digit tips.
DelEC1	E15.5	TS23: forelimb digit	Present (a)	0.5 µg; total RNA	involves: C57BL/6 * CBA	Hoxc^em1Ddu/Hoxc^em1Ddu	Not Specified	Sample was forelimb digit tips.
DelEC2	E15.5	TS23: forelimb digit	Present (b)	0.5 µg; total RNA	involves: C57BL/6 * CBA	Hoxc^em2Ddu/Hoxc^em2Ddu	Not Specified	Sample was forelimb digit tips.
DelEC1-EC2	E15.5	TS23: forelimb digit	Present (c)	0.5 µg; total RNA	involves: C57BL/6 * CBA	Hoxc^em3Ddu/Hoxc^em3Ddu	Not Specified	Sample was forelimb digit tips.
TH	E15.5	TS23: forelimb digit	Present (d)	0.5 µg; total RNA	involves: 129S/SvEv * C57BL/6 * CBA	Hoxc^em3Ddu/Hoxc^tm1Nogu	Not Specified	Sample was forelimb digit tips.
HoxCKO	E15.5	TS23: forelimb digit	Absent	0.5 µg; total RNA	involves: 129S/SvEv * C57BL/6	Hoxc^tm1Nogu/Hoxc^tm1Nogu	Not Specified	Sample was forelimb digit tips.

Notes:
(a) HoxC<delEC1> homozygous expression level was decreased 40% compared to wild type.
(b) HoxC<delEC2> homozygous expression level was only 30% of the total amount of Hoxc13 mRNAs.
(c) HoxC<del(EC1-EC2)> homozygous expression level was only 5% of the total amount of Hoxc13 mRNAs.
(d) HoxC<del(EC1-EC2)/-> transheterozygous expression level was only 2% of the total amount of Hoxc13 mRNAs.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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