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Gene Expression Data
Assay Details
Assay
Reference: J:297683 Fernandez-Guerrero M, et al., Mammalian-specific ectodermal enhancers control the expression of Hoxc genes in developing nails and hair follicles. Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30509-30519
Assay type: RT-PCR
MGI Accession ID: MGI:7335083
Gene symbol: Hoxc13
Gene name: homeobox C13
Probe: Hoxc13-pK, Hoxc13-pL
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh. The level of expression for wild type control was set to 1.
Results
Image: 6 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Wt E15.5 TS23: forelimb digit Present 0.5 µg; total RNA Not Specified Not Specified Sample was forelimb digit tips.
Wt E15.5 TS23: forelimb digit Present 0.5 µg; total RNA Not Specified Not Specified Sample was forelimb digit tips.
HoxCKO Het E15.5 TS23: forelimb digit Present 0.5 µg; total RNA involves: 129S/SvEv * C57BL/6 Hoxctm1Nogu/Hoxc+Not Specified Sample was forelimb digit tips.
DelEC1 E15.5 TS23: forelimb digit Present (a) 0.5 µg; total RNA involves: C57BL/6 * CBA Hoxcem1Ddu/Hoxcem1DduNot Specified Sample was forelimb digit tips.
DelEC2 E15.5 TS23: forelimb digit Present (b) 0.5 µg; total RNA involves: C57BL/6 * CBA Hoxcem2Ddu/Hoxcem2DduNot Specified Sample was forelimb digit tips.
DelEC1-EC2 E15.5 TS23: forelimb digit Present (c) 0.5 µg; total RNA involves: C57BL/6 * CBA Hoxcem3Ddu/Hoxcem3DduNot Specified Sample was forelimb digit tips.
TH E15.5 TS23: forelimb digit Present (d) 0.5 µg; total RNA involves: 129S/SvEv * C57BL/6 * CBA Hoxcem3Ddu/Hoxctm1NoguNot Specified Sample was forelimb digit tips.
HoxCKO E15.5 TS23: forelimb digit Absent 0.5 µg; total RNA involves: 129S/SvEv * C57BL/6 Hoxctm1Nogu/Hoxctm1NoguNot Specified Sample was forelimb digit tips.
Notes:
(a) HoxC<delEC1> homozygous expression level was decreased 40% compared to wild type.
(b) HoxC<delEC2> homozygous expression level was only 30% of the total amount of Hoxc13 mRNAs.
(c) HoxC<del(EC1-EC2)> homozygous expression level was only 5% of the total amount of Hoxc13 mRNAs.
(d) HoxC<del(EC1-EC2)/-> transheterozygous expression level was only 2% of the total amount of Hoxc13 mRNAs.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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