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Gene Expression Data
Assay Details
Assay
Reference: J:203995 Lee S, et al., Forward genetics identifies Kdf1/1810019J16Rik as an essential regulator of the proliferation-differentiation decision in epidermal progenitor cells. Dev Biol. 2013 Nov 15;383(2):201-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7333007
Gene symbol: Krt10
Gene name: keratin 10
Antibody: Anti-K10
Results
Specimen 2B control: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present 2B left
TS26: epidermis basal layer Absent 2B left
Specimen 2B -/-: embryonic day 18.5; Kdf1shd/Kdf1shd (more )
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present Regionally restricted 2B right Expression domain was expanded distally, and overlapped with basal marker expression.
TS26: epidermis basal layer Absent 2B right
Specimen 2E control: embryonic day 18.5 (more )
Note: Double labeled: red - Krt10; green - Mki67 (assay MGI:7333010).
Structure Cell Type Level Pattern Image Note
TS26: epidermis suprabasal layer keratinocyte Present 2E left
TS26: epidermis basal layer Absent 2E left
Specimen 2E -/-: embryonic day 18.5; Kdf1shd/Kdf1shd (more )
Note: Double labeled: red - Krt10; green - Mki67 (assay MGI:7333010).
Structure Cell Type Level Pattern Image Note
TS26: epidermis suprabasal layer keratinocyte Present Regionally restricted 2E right Expression domain was expanded distally.
TS26: epidermis basal layer Absent 2E right
Specimen 3G control: embryonic day 18.5 (more )
Note: Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present 3G left
TS26: epidermis basal layer Absent 3G left
Specimen 3G shd+/-; Er+/-: embryonic day 18.5; Kdf1shd/Kdf1+, SfnEr/Sfn+ (more )
Note: Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Present Regionally restricted 3G right The epidermis was greatly thickened and the cornified layer displayed gaps, with positive keratinocytes spilling out onto the surface.
TS26: epidermis basal layer Absent 3G right
Specimen 4I control: embryonic day 18.5 (more )
Note: Lateral back skin. Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present 4I left
Specimen 4I shd-/-; p63+/-: embryonic day 18.5; Kdf1shd/Kdf1shd, Trp63tm2Brd/Trp63+ (more )
Note: Lateral back skin. Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present 4I right Normal thickness and expression was restored in lateral (barrier-restored) regions.
Specimen 6C control: embryonic day 18.5 (more )
Note: Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis spinous layer keratinocyte Present 6C left
Specimen 6C GT/shd: embryonic day 18.5; Kdf1shd/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Present Regionally restricted 6C middle Expression domain was expanded distally.
TS26: epidermis basal layer Absent 6C middle
Specimen 6C GT/GT: embryonic day 18.5; Kdf1tm1a(EUCOMM)Wtsi/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Krt10; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Present Regionally restricted 6C right Expression domain was expanded distally.
TS26: epidermis basal layer Absent 6C right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory