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Gene Expression Data
Assay Details
Assay
Reference: J:216287 Czajkowski MT, et al., Divergent and conserved roles of Dll1 signaling in development of craniofacial and trunk muscle. Dev Biol. 2014 Nov 15;395(2):307-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7331677
Gene symbol: Myh2
Gene name: myosin, heavy polypeptide 2, skeletal muscle, adult
Antibody: clone MY-32 (M4276)
Detection system: Secondary antibody
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 2A/E: embryonic day 13.0; Dll1+/Dll1tm2Gos (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS21: cranial muscle Present Regionally restricted 2A , 2E Expressed in the masseter.
TS21: extrinsic ocular pre-muscle mass Present 2A
TS21: tongue muscle Present 2A
Specimen 2B/F: embryonic day 13.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS21: cranial muscle Present Regionally restricted 2B , 2F Expressed in the masseter. The size was clearly reduced compared with the control.
TS21: extrinsic ocular pre-muscle mass Present 2B Similar as in the control.
TS21: tongue muscle Present 2B Similar as in the control.
Specimen 2C/G: embryonic day 18.0; Dll1+/Dll1tm2Gos (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS26: extrinsic ocular muscle Present 2G , 2C
TS26: masseter Present 2G , 2C
TS26: tongue muscle Present 2G , 2C
Specimen 2D/H: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS26: masseter Trace 2D , 2H Severely reduced in size.
TS26: extrinsic ocular muscle Trace 2D Severely reduced in size.
TS26: tongue muscle Present 2D Reduced in size but appeared less strongly affected than other craniofacial muscle groups.
Specimen 5A: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS26: extrinsic ocular muscle Trace 5A Severely reduced in size.
TS26: masseter Trace 5A Severely reduced in size.
TS26: tongue extrinsic muscle Present 5A Ex , 5A Reduced in size but appeared less strongly affected than other craniofacial muscle groups.
TS26: tongue intrinsic muscle Present 5A In , 5A Reduced in size but appeared less strongly affected than other craniofacial muscle groups.
Specimen 5B: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS26: extrinsic ocular muscle Present 5B Markedly increased in the size of the muscle compared to Dll1<LacZ/Ki> mice, similar as in MyoD-/- mice.
TS26: masseter Present 5B Markedly increased in the size of the muscle compared to Dll1<LacZ/Ki> mice, similar as in MyoD-/- mice.
TS26: tongue extrinsic muscle Present 5B Ex , 5B Markedly increased in the size of the muscle compared to Dll1<LacZ/Ki> mice, similar as in MyoD-/- mice.
TS26: tongue intrinsic muscle Present 5B , 5B In Markedly increased in the size of the muscle compared to Dll1<LacZ/Ki> mice, similar as in MyoD-/- mice.
Specimen 5C: embryonic day 18.0; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: red - Myh1 and Myh2; green - Pax7 (assay MGI:7331535).
Structure Level Pattern Image Note
TS26: extrinsic ocular muscle Present 5C
TS26: masseter Present 5C
TS26: tongue extrinsic muscle Present 5C , 5C Ex
TS26: tongue intrinsic muscle Present 5C , 5C In
Specimen not shown: embryonic day 12.0; Dll1+/Dll1tm2Gos (more )
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present
TS20: tongue muscle Present
TS20: face mesenchyme Present Regionally restricted Expressed in the masseter.
Specimen not shown: embryonic day 12.0; Dll1tm1Gos/Dll1tm2Gos (more )
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present Similar as in the control.
TS20: tongue muscle Present Similar as in the control.
TS20: face mesenchyme Present Regionally restricted Expressed in the masseter, similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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