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Gene Expression Data
Assay Details
Assay
Reference: J:216287 Czajkowski MT, et al., Divergent and conserved roles of Dll1 signaling in development of craniofacial and trunk muscle. Dev Biol. 2014 Nov 15;395(2):307-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7331535
Gene symbol: Pax7
Gene name: paired box 7
Antibody: Anti-Pax7
Detection system: Secondary antibody
Results
Specimen 2A/E: embryonic day 13.0; Dll1+/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS21: extrinsic ocular pre-muscle mass muscle precursor cell Present 2E , 2A
TS21: tongue muscle muscle precursor cell Present 2E , 2A
TS21: cranial muscle muscle precursor cell Present Regionally restricted 2A Expressed in the masseter.

Specimen 2B/F: embryonic day 13.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS21: cranial muscle muscle precursor cell Absent 2B , 2F
TS21: extrinsic ocular pre-muscle mass muscle precursor cell Absent 2B
TS21: tongue muscle muscle precursor cell Absent 2B

Specimen 2C/G: embryonic day 18.0; Dll1+/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS26: masseter muscle precursor cell Present 2C , 2G
TS26: extrinsic ocular muscle muscle precursor cell Present 2C
TS26: tongue muscle muscle precursor cell Present 2C

Specimen 2D/H: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS26: masseter muscle precursor cell Absent 2D , 2H
TS26: extrinsic ocular muscle muscle precursor cell Absent 2D
TS26: tongue muscle muscle precursor cell Absent 2D

Specimen 5A: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS26: extrinsic ocular muscle muscle precursor cell Absent 5A
TS26: masseter muscle precursor cell Absent 5A
TS26: tongue extrinsic muscle muscle precursor cell Absent 5A , 5A Ex
TS26: tongue intrinsic muscle muscle precursor cell Absent 5A In , 5A

Specimen 5B: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS26: tongue intrinsic muscle muscle precursor cell Trace Single cells 5B In , 5B Expressed in only few cells.
TS26: tongue extrinsic muscle muscle precursor cell Present 5B Ex , 5B Positive cells were abundant.
TS26: extrinsic ocular muscle muscle precursor cell Present 5B The rescue of the progenitor pool was extensive but not complete.
TS26: masseter muscle precursor cell Present 5B Similar as in the MyoD-/-.

Specimen 5C: embryonic day 18.0; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Myh1 (assay MGI:7331537) and Myh2 (assay MGI:7331677).
Structure Cell Type Level Pattern Image Note
TS26: tongue extrinsic muscle muscle precursor cell Present 5C , 5C Ex
TS26: tongue intrinsic muscle muscle precursor cell Present 5C In , 5C
TS26: extrinsic ocular muscle muscle precursor cell Present 5C
TS26: masseter muscle precursor cell Present 5C

Specimen 5D: embryonic day 18.0 (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: masseter muscle precursor cell Present 5D

Specimen 5E: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Level Pattern Image Note
TS26: masseter Absent 5E

Specimen 5F: embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: masseter muscle precursor cell Present 5F Number of positive cells was higher than controls and similar as in MyoD-/- (summarized in 5H).

Specimen 5G: embryonic day 18.0; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: masseter muscle precursor cell Present 5G Number of positive cells was higher than controls (summarized in 5H).

Specimen 5D': embryonic day 18.0 (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: tongue extrinsic muscle muscle precursor cell Present 5D'

Specimen 5E': embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: tongue extrinsic muscle muscle precursor cell Absent 5E'

Specimen 5F': embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: tongue extrinsic muscle muscle precursor cell Present 5F' Number of positive cells was higher than controls and similar as in MyoD-/- (summarized in 5H').

Specimen 5G': embryonic day 18.0; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Level Pattern Image Note
TS26: tongue extrinsic muscle Present 5G' Number of positive cells was higher than controls (summarized in 5H).

Specimen 5D": embryonic day 18.0 (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: extrinsic ocular muscle muscle precursor cell Present 5D"

Specimen 5E": embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Level Pattern Image Note
TS26: extrinsic ocular muscle Absent 5E"

Specimen 5F": embryonic day 18.0; Dll1tm1Gos/Dll1tm2Gos, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: extrinsic ocular muscle muscle precursor cell Present 5F" The rescue of the progenitor pool was extensive but not complete (summarized in 5H").

Specimen 5G": embryonic day 18.0; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; blue - Myh1 (assay MGI:7331550 and Myh2 (assay MGI:7331680).
Structure Cell Type Level Pattern Image Note
TS26: extrinsic ocular muscle muscle precursor cell Present 5G" Number of positive cells was higher than controls (summarized in 5H").


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory