Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42759
Panning B, et al., X chromosome inactivation is mediated by Xist RNA stabilization. Cell. 1997 Sep 5;90(5):907-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7331495
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Gene symbol:
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Xist
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Gene name:
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inactive X specific transcripts
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Results
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Specimen
1B:
embryonic day 3.5
(more  )
Note: Fixation - methanol:acetic acid.
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Male
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Fixation - methanol:acetic acid.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Weak |
Punctate |
1B
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Male inner cell mass preparations showed a pattern of low level, dot-like expression.
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Specimen
1E:
embryonic day 3.5
(more )
Note: Fixation - methanol:acetic acid.
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Female
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Fixation - methanol:acetic acid.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Weak |
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1E
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Female inner cell mass preparations showed biallelic expression.
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Specimen
Female ICM:
embryonic day 3.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female ICM:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Weak |
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Inner cell mass derived cells showed low level biallelic expression.
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Specimen
Female E6.5:
embryonic day 6.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E6.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
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Female embryos showed three patterns of Xist expression, the proportion of which changed over time. At E6.5, 1. a small fraction of the cells showed low level biallelic Xist expression. 2. a slightly larger proportion of cells displayed differential biallelic Xist expression. 3. in the majority of cells, abundant Xist RNA was detected from only one locus.
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Specimen
Female E7.0:
embryonic day 7.0
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E7.0:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Present |
Regionally restricted |
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Female embryos showed three patterns of Xist expression, the proportion of which changed over time. At E7.0, 1. a small fraction of the cells showed low level biallelic Xist expression. 2. a slightly larger proportion of cells displayed differential biallelic Xist expression. 3. in the majority of cells, abundant Xist RNA was detected from only one locus.
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Specimen
Female E7.5:
embryonic day 7.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E7.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
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In female embryos at E7.5 cells no longer showed low level biallelic Xist expression. A proportion of cells displayed differential biallelic Xist expression, and in the majority of cells, abundant Xist RNA was detected from only one locus.
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Specimen
Female E8.0:
embryonic day 8.0
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E8.0:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
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Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
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In female embryos at E8.0 a proportion of cells displayed differential biallelic Xist expression, but in the majority of cells abundant Xist RNA was detected from only one locus.
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Specimen
Female E8.5:
embryonic day 8.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E8.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Regionally restricted |
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In female embryos at E8.5 abundant Xist RNA was detected from only one locus.
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Specimen
Male ICM:
embryonic day 3.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male ICM:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Male
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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|
Note:
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Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Weak |
Regionally restricted |
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Low level monoallelic expression was detected in males.
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Specimen
Male E6.5:
embryonic day 6.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E6.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Weak |
Regionally restricted |
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In males, two patterns of expression could be discerned. Low level monoallelic expression or cells didn't express any detectable Xist RNA. The proportion of cells with low level expression decreased over time.
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Specimen
Male E7.0:
embryonic day 7.0
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E7.0:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Sex:
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Male
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Type:
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section
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Fixation:
|
Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Weak |
Regionally restricted |
|
In males, two patterns of expression could be discerned. Low level monoallelic expression or cells didn't express any detectable Xist RNA. The proportion of cells with low level expression decreased over time.
|
Specimen
Male E7.5:
embryonic day 7.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E7.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Male
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Type:
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section
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Fixation:
|
Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Weak |
Regionally restricted |
|
In males, two patterns of expression could be discerned. Low level monoallelic expression or cells didn't express any detectable Xist RNA. The proportion of cells with low level expression decreased over time.
|
Specimen
Male E8.0:
embryonic day 8.0
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E8.0:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Male
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Type:
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section
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Fixation:
|
Paraformaldehyde
|
|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Weak |
Regionally restricted |
|
In males, two patterns of expression could be discerned. Low level monoallelic expression or cells didn't express any detectable Xist RNA. The proportion of cells with low level expression decreased over time.
|
Specimen
Male E8.5:
embryonic day 8.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E8.5:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 8.5
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|
Sex:
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Male
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Type:
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section
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Fixation:
|
Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Absent |
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The proportion of cells with low level Xist expression was no longer present at E8.5.
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Specimen
Female E3.5 trophoblast:
embryonic day 3.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female E3.5 trophoblast:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Female
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Present |
Regionally restricted |
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All female trophectodermal cells showed differential biallelic expression.
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Specimen
Female extraembryonic E7.5:
embryonic day 7.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female extraembryonic E7.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: extraembryonic component |
Present |
Regionally restricted |
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In E7.5 female extraembryonic component, 5% of cells showed a differential biallelic pattern, and the remainder showed a high level monoallelic pattern.
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Specimen
Female extraembryonic E8.5:
embryonic day 8.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Female extraembryonic E8.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
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Presented in Fig. 2 graphs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: extraembryonic component |
Present |
Widespread |
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In E8.5 female extraembryonic component showed a high level monoallelic pattern.
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Specimen
Male E3.5 trophoblast:
embryonic day 3.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male E3.5 trophoblast:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Male
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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|
Note:
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Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Weak |
Regionally restricted |
|
Male trophoblast cells showed low level monoallelic expression.
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Specimen
Male extraembryonic E7.5:
embryonic day 7.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male extraembryonic E7.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
|
Presented in Fig. 2 graphs.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: extraembryonic component |
Weak |
Regionally restricted |
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Male extraembryonic cells showed low level monoallelic or no Xist expression.
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Specimen
Male extraembryonic E8.5:
embryonic day 8.5
(more )
Note: Presented in Fig. 2 graphs.
Specimen
Male extraembryonic E8.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Paraformaldehyde
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|
Note:
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Presented in Fig. 2 graphs.
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Analysis Tools