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Gene Expression Data
Assay Details
Assay
Reference: J:320689 Xu J, et al., Six1 and Six2 of the Sine Oculis Homeobox Subfamily are Not Functionally Interchangeable in Mouse Nephron Formation. Front Cell Dev Biol. 2022;10:815249
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7328529
Gene symbol: Six1
Gene name: sine oculis-related homeobox 1
Antibody: anti-SIX1 (D4A8K; #12891)
Detection system: Secondary antibody coupled to Cy5
Results
Specimen 5B +/+: embryonic day 12.5 (more )
Note: Double labeled: red - Six1; green - Eya1 (assay MGI:7328528).
Structure Level Pattern Image Note
TS20: cap mesenchyme Absent 5B +/+ lower , 5B +/+ upper
TS20: renal vesicle Absent 5B +/+ lower , 5B +/+ upper
Specimen 5B Six1/+: embryonic day 12.5; Eya1tm4(Six1)Px/Eya1+ (more )
Note: Double labeled: red - Six1; green - Eya1 (assay MGI:7328528).
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 5B Six1/+ lower , 5B Six1/+ upper Coexpressed with Eya1.
TS20: renal vesicle Present 5B Six1/+ lower , 5B Six1/+ upper Coexpressed with Eya1.
Specimen 5B Six1/+;Six2-/-: embryonic day 12.5; Eya1tm4(Six1)Px/Eya1+, Six2tm1Gco/Six2tm1Gco (more )
Note: Double labeled: red - Six1; green - Eya1 (assay MGI:7328528).
Structure Level Pattern Image Note
TS20: cap mesenchyme Absent 5B Six1/+; Six2-/- upper , 5B Six1/+; Six2-/- lower
TS20: renal vesicle Absent 5B Six1/+; Six2-/- upper , 5B Six1/+; Six2-/- lower
Specimen 6A +/+: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cap mesenchyme Absent 6A Six1 +/+
Specimen 6A Six1/+: embryonic day 16.5; Eya1tm4(Six1)Px/Eya1+ (more )
Structure Level Pattern Image Note
TS24: cap mesenchyme Strong 6A Six1 Six1/+
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory