Eya1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:320689 Xu J, et al., Six1 and Six2 of the Sine Oculis Homeobox Subfamily are Not Functionally Interchangeable in Mouse Nephron Formation. Front Cell Dev Biol. 2022;10:815249
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7328528
Gene symbol:	Eya1
Gene name:	EYA transcriptional coactivator and phosphatase 1

Antibody:	Anti-Eyes absent homolog 1 Antibody, clone 6A3.1 (MABE1047)
Detection system:	Secondary antibody coupled to Cy2

Results

5B +/+ lower ©

5B +/+ upper ©

Specimen 5B +/+: embryonic day 12.5 (more

)
Note: Double labeled: green - Eya1; red - Six1 (assay MGI:7328529).

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present		5B +/+ lower , 5B +/+ upper	Higher levels.
TS20: renal vesicle	Present		5B +/+ upper	Lower levels.

Specimen 5B Six1/+: embryonic day 12.5; Eya1^tm4(Six1)Px/Eya1⁺ (more

)
Note: Double labeled: green - Eya1; red - Six1 (assay MGI:7328529).

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present		5B Six1/+ lower , 5B Six1/+ upper	Higher levels. Coexpressed with Six1.
TS20: renal vesicle	Present		5B Six1/+ upper	Lower levels. Coexpressed with Six1.

Specimen 5B Six1/+;Six2-/-: embryonic day 12.5; Eya1^tm4(Six1)Px/Eya1⁺, Six2^tm1Gco/Six2^tm1Gco (more

)
Note: Double labeled: green - Eya1; red - Six1 (assay MGI:7328529).

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Absent		5B Six1/+; Six2-/- lower , 5B Six1/+; Six2-/- upper

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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