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Gene Expression Data
Assay Details
Assay
Reference: J:320689 Xu J, et al., Six1 and Six2 of the Sine Oculis Homeobox Subfamily are Not Functionally Interchangeable in Mouse Nephron Formation. Front Cell Dev Biol. 2022;10:815249
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7328521
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Secondary antibody coupled to Cy5
Results
Specimen 1C +/+: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: cap mesenchyme Present 1D +/+ right , 1D +/+ left Expressed in the nephron progenitors.
TS22: pretubular aggregate Present 1D +/+ left , 1D +/+ right
TS22: renal vesicle Present 1D +/+ left , 1D +/+ right
TS22: capillary loop renal corpuscle podocyte Present 1D +/+ left , 1D +/+ right Expressed in differentiating podocytes.
Specimen 1C Six1/+: embryonic day 14.5; Eya1tm4(Six1)Px/Eya1+ (more )
Structure Cell Type Level Pattern Image Note
TS22: cap mesenchyme Present 1D Six1/+ right , 1D Six1/+ left Expressed in the nephron progenitors, similar as in the wild-type.
TS22: pretubular aggregate Present 1D Six1/+ right , 1D Six1/+ left Similar as in the wild-type.
TS22: renal vesicle Present 1D Six1/+ right , 1D Six1/+ left Similar as in the wild-type.
TS22: capillary loop renal corpuscle podocyte Present 1D Six1/+ left , 1D Six1/+ right Expressed in differentiating podocytes, similar as in the wild-type.
Specimen 1C Six1/+;Six2-/-: embryonic day 14.5; Eya1tm4(Six1)Px/Eya1+, Six2tm1Gco/Six2tm1Gco (more )
Structure Level Pattern Image Note
TS22: cap mesenchyme Ambiguous 1D Six1/+, Six2-/- left , 1D Six1/+, Six2-/- right Lacked the expression in nephron progenitors in the outmost nephrogenic zone.
Specimen 1C Six2-/-: embryonic day 14.5; Six2tm1Gco/Six2tm1Gco (more )
Structure Level Pattern Image Note
TS22: cap mesenchyme Ambiguous 1D Six2-/- left , 1D Six2-/- right Lacked the expression in nephron progenitors in the outmost nephrogenic zone.
Specimen 2I: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 2I
TS20: renal vesicle Present 2I
Specimen 2J: embryonic day 12.5; Eya1tm4(Six1)Px/Eya1+ (more )
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 2J
TS20: renal vesicle Present 2J
Specimen 2K: embryonic day 12.5; Eya1tm4(Six1)Px/Eya1+, Six2tm1Gco/Six2tm1Gco (more )
Structure Level Pattern Image Note
TS20: mesenchyme of metanephros Present Regionally restricted 2K Expressed in metanephric mesenchyme progenitors surrounding the branching ureteric bud, which did not commit to an overall renal vesicle fate.
Specimen 2L: embryonic day 12.5; Six2tm1Gco/Six2tm1Gco (more )
Structure Level Pattern Image Note
TS20: mesenchyme of metanephros Present Regionally restricted 2L Expressed in renal vesicle-like structures surrounded the entire ureteric bud.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory