Ar Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:324263 Aksel S, et al., Ontogeny of mouse Sertoli, Leydig and peritubular myoid cells from embryonic day 10 to adulthood. Differentiation. 2022 Mar 7;
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7314197
Gene symbol:	Ar
Gene name:	androgen receptor

Antibody:	AR antibody (EPR1535)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 11A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: gonad	Absent		11A
TS19: mesonephric tubule	Present		11A

Specimen 11B: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: mesonephros mesenchyme	Present		11B
TS19: nephric duct	Absent		11B

Specimen 12A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: interstitium of the testis	Present	Regionally restricted	12A	Expression in a subset of interstitial cells scattered between testicular cords.
TS20: mesonephros	Present	Regionally restricted	12A	Expression at mesonephric-gonadal border
TS20: mesonephros mesenchyme	Present		12A

Specimen 12B: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: interstitium of the testis	Present	Graded	12B	Expression in interstitial cells more abundant between testicular cords, likely pertibular myeloid cell precursors. Higher percent of expressing cells near mesonephros; lower percentage near coelomic border.

Specimen 12C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Regionally restricted	12C	Expressing cells intimately associated with testicular cords and seminiferous tubules in positions indicative of peritubular myeloic cells,
TS22: interstitium of the testis	Present	Regionally restricted	12C	Expression throughtout the interstitium in positions indicative of Leydig cells.

Specimen 12D: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: peritubular myoid cell	peritubular myoid cell	Present		12D
TS24: Leydig cell	Leydig cell	Present		12D	Expression in interstitium, likely Leydig cells.

Specimen 12E: postnatal newborn (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: peritubular myoid cell	peritubular myoid cell	Present		12E
TS27: Leydig cell	Leydig cell	Present		12E	v

Specimen 12F: postnatal day 14 (more

)

Structure	Cell Type	Level	Image	Note
TS28: peritubular myoid cell	peritubular myoid cell	Present	12F
TS28: Leydig cell	Leydig cell	Present	12F	Expression in interstitium, likely Leydig cells.
TS28: Sertoli cell	Sertoli cell	Weak	12F

Specimen 12G: postnatal day 60 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: peritubular myoid cell	peritubular myoid cell	Present		12G
TS28: Sertoli cell	Sertoli cell	Weak		12G

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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