Gene Expression Data
Assay Details
Assay
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Reference:
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J:320687
Sheban D, et al., SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity. Mol Cell. 2022 Jan 6;82(1):106-122.e9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7311801
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Gene symbol:
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Sumo2
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Gene name:
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small ubiquitin-like modifier 2
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Results
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Specimen
1A 1-Cell SUMO2/3:
embryonic day 0.5
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A 1-Cell SUMO2/3:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Absent |
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1A 1-Cell Merge
,
1A 1-Cell SUMO2/3
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Expression nearly undetectable at 1-cell through 4-cell stages.
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Specimen
1A 4-Cell SUMO2/3:
embryonic day 2.0
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A 4-Cell SUMO2/3:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Absent |
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1A 4-Cell Merge
,
1A 4-Cell SUMO2/3
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Expression nearly undetectable at 1-cell through 4-cell stages.
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Specimen
1A 8-Cell SUMO2/3:
embryonic day 2.5
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A 8-Cell SUMO2/3:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 2.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
|
Specimen
1A 16-Cell SUMO2/3:
embryonic day 3.0
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A 16-Cell SUMO2/3:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
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Specimen
1A Morula SUMO2/3:
embryonic day 3.0
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A Morula SUMO2/3:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Strong |
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1A Morula Merge
,
1A Morula SUMO2/3
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Expression highly abundant at morula and blastocyst stages.
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Specimen
1A Blastocyst SUMO2/3:
embryonic day 3.5
(more )
Note: Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
Specimen
1A Blastocyst SUMO2/3:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sumo2 and Sumo3; green - OCT4 (Pou5f1 assay MGI:7311796).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Strong |
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1A Blastocyst SUMO2/3
,
1A Blastocyst Merge
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Expression highly abundant at morula and blastocyst stages.
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