Gene Expression Data
Assay Details
Assay
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Reference:
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J:320687
Sheban D, et al., SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity. Mol Cell. 2022 Jan 6;82(1):106-122.e9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7311796
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Results
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Specimen
1A 1-Cell OCT4:
embryonic day 0.5
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A 1-Cell OCT4:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Ambiguous |
|
1A 1-Cell OCT4
,
1A 1-Cell Merge
|
Oct4 expression not specifically described.
|
Specimen
1A 4-Cell OCT4:
embryonic day 2.0
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A 4-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
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Age:
|
embryonic day 2.0
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Ambiguous |
|
1A 4-Cell OCT4
,
1A 4-Cell Merge
|
Oct4 expression not specifically described.
|
Specimen
1A 8-Cell OCT4:
embryonic day 2.5
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A 8-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 2.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
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whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Specimen
1A 16-Cell OCT4:
embryonic day 3.0
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A 16-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Specimen
1A Morula OCT4:
embryonic day 3.0
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A Morula OCT4:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Specimen
1A Blastocyst OCT4:
embryonic day 3.5
(more )
Note: Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
Specimen
1A Blastocyst OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
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Age:
|
embryonic day 3.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Pou5f1; red - Sumo2 (assay MGI:7311801) and Sumo3 (assay MGI:7311805).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Strong |
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1A Blastocyst OCT4
,
1A Blastocyst Merge
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Specimen
S1A 1-Cell OCT4:
embryonic day 0.5
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A 1-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 0.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Ambiguous |
|
S1A 1-Cell Merge
,
S1A 1-Cell OCT4
|
Oct4 expression not specifically described.
|
Specimen
S1A 4-Cell OCT4:
embryonic day 2.0
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A 4-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 2.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Ambiguous |
|
S1A 4-Cell Merge
,
S1A 4-Cell OCT4
|
Oct4 expression not specifically described.
|
Specimen
S1A 8-Cell OCT4:
embryonic day 2.5
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A 8-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 2.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage conceptus |
Ambiguous |
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S1A 8-Cell OCT4
,
S1A 8-Cell Merge
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Oct4 expression not specifically described.
|
Specimen
S1A 16-Cell OCT4:
embryonic day 3.0
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A 16-Cell OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Specimen
S1A Morula OCT4:
embryonic day 3.0
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A Morula OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Specimen
S1A Blastocyst OCT4:
embryonic day 3.5
(more )
Note: Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
Specimen
S1A Blastocyst OCT4:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA/2
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - OCT4 (Pou5f1); red - Sumo1 (assay MGI:7311798).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Strong |
|
S1A Blastocyst Merge
,
S1A Blastocyst OCT4
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