Probe:	Fzd1 cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		1A
TS15: diencephalon	Weak	Uniform	1A
TS15: future hindbrain	Weak	Uniform	1A
TS15: future midbrain	Weak	Uniform	1A
TS15: telencephalon	Weak	Uniform	1A

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	2A	Expression is in the dorsal telencephalon, extending in a gradient both anteriorly into the rostral telencephalon and posteriorly to the telencephalic/diencephalic boundary. There is also ventral expression.
TS17: head mesenchyme	Strong	Regionally restricted	2A	Expression is in the rostral head mesenchyme.
TS17: hindbrain	Present	Regionally restricted	2A	Expression is ventral and is is very low in the dorsal part of the anterior rhombencephalon.
TS17: diencephalon	Present	Regionally restricted	2A	Expression is mostly ventral and is very low in the dorsal part of the anterior diencephalon. There is a dorsal boundary and ventral gap in expression corresponding to the position of the zona limitans intrathalamica.
TS17: midbrain	Present	Regionally restricted	2A	Expression is mostly ventral and is very low in the dorsal part of the anterior midbrain. There is a gap in expression in the caudal cephalic flexure/ventro-caudal mesencephalon, extending just rostral to the isthmus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	3A	Expression is in the hippocampal anlage, septal area.
TS20: cerebral cortex	Strong		3A
TS20: ganglionic eminence	Strong		3A
TS20: mammillary area	Strong		3A
TS20: pons	Strong		3A
TS20: preoptic area	Strong		3A
TS20: head mesenchyme	Strong		3A
TS20: diencephalon	Strong	Regionally restricted	3A	Expression is in the optic recess, postoptic area, subthalamic area (weaker in a patch within the subthalamic area), and the cephalic flexure.
TS20: hindbrain	Strong	Regionally restricted	3A	Expression is ventral.
TS20: thalamus	Weak	Regionally restricted	3A	Expression is in the dorsal thalamus
TS20: midbrain roof plate	Weak	Regionally restricted	3A	Expression is in the tectum and is absent in the pretectum and the most caudal part of the tectum.
TS20: cerebellum primordium	Weak		3A
TS20: midbrain	Present	Regionally restricted	3A	Expression is absent from the pretectum and the most caudal part of the tectum There is an expression gap in the ventral midbrain approximating the position of the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4B	Expression is in the ventral part of the anterior hindbrain (rhombomere 1, r1).
TS17: midbrain	Present	Regionally restricted	4B	Expression is ventral and trace expression in the dorsal midbrain. There is a gap in expression along the anterior/posterior axis of the caudal ventral midbrain corresponding to the caudal cephalic flexure.
TS17: midbrain-hindbrain junction	Trace		4B

Structure	Level	Pattern	Image	Note
TS17: midbrain roof plate	Present		4B'
TS17: midbrain ventricular layer	Weak	Regionally restricted	4B'	Expression is throughout the dorsal/ventral extent.
TS17: midbrain lateral wall	Weak	Regionally restricted	4B'	Expression is throughout the dorsal/ventral extent of the subventricular zone.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5C	There is a gap in expression in the caudal cephalic flexure/ventro-caudal mesencephalon, extending just rostral to the isthmus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	6B	Expression is in the ventral midline of the rostral hindbrain (r1).
TS20: midbrain	Strong	Regionally restricted	6B	Expression is in the ventral midline of the caudal midbrain with lower expression along the anterior/posterior axis of the caudal cephalic flexure. Expression is in the rostral tectum and is lower in the caudal tectum.
TS20: cerebellum primordium	Strong		6B

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	6B'	Expression is throughout the neuroepithelium of the alar plate with a sharp ventral border at approximately the position of the alar/basal boundary. Expression is confined mostly to the ventricular zone/subventricular zone of the midbrain basal and floor plate except a small transverse stripe just below the alar/basal boundary where its expression extends into the marginal zone.
TS20: midbrain floor plate	Strong	Regionally restricted	6B'
TS20: midbrain marginal layer	Strong	Regionally restricted	6B'
TS20: midbrain ventricular layer	Strong	Regionally restricted	6B'
TS20: midbrain roof plate	Present	Regionally restricted	6B'	Expression is in the rostral tectum and at lower levels in the caudal tectum.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	8A	Expression is in the pallium and subpallium, with strongest expression in the dorsal and medial pallium.
TS20: cerebral cortex	Strong		8A
TS20: cortical hem	Strong		8A
TS20: preoptic area	Strong		8A
TS20: lateral ganglionic eminence	Strong	Regionally restricted	8A	Expression is in the ventricular and subventricular zone.
TS20: medial ganglionic eminence	Strong	Regionally restricted	8A	Expression is in the ventricular and subventricular zone.
TS20: choroid plexus	Present	Regionally restricted	8A	Expression is in the epithelial layer.
TS20: diencephalon	Present	Regionally restricted	8A	Expression is in the thalamic eminence.
TS20: thalamus	Weak	Regionally restricted	8A	Expression is in the dorsal thalamus.