Antibody:	anti-SSEA-1
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1A	Expression predominantly along ventricular lining of diencephalon, along surface of third ventricle.
TS15: optic recess	Present		1A	Expression in lumen of optic stalk.
TS15: Rathke's pouch lumen	Strong		1A	Strong expression in lumen of Rathke's pouch.
TS15: infundibular recess of 3rd ventricle	Absent		1A	No expression in infundibular stalk.

Structure	Level	Pattern	Image	Note
TS17: diencephalon ventricular layer	Strong	Regionally restricted	1B	Intense expression restricted to ventricular layer of caudal parts of ventral diencephalon. Weak expression in other diencephalic regions. Weak expression in intraretinal space.
TS17: Rathke's pouch	Weak		1B

Structure	Level	Pattern	Image	Note
TS19: diencephalon ventricular layer	Strong	Regionally restricted	1C	TS19; diencephalon ventricular layer
TS19: diencephalon lateral wall	Present	Regionally restricted	1C	Expression in subventricular zone of diencephalon in caudal regions of ventral diencephalon - appear to link with expression on ventricular surface.
TS19: forebrain	Present	Regionally restricted	1C	Intense expression at junction of diencephalon and telencephalon.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall	Strong	Regionally restricted	1D	Intense expression within subventricular zone of diencephalon.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1E	Expression localized in two symmetrical domains in caudal regions of diencephalon.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1F	Expression in cell bodies and processes of chiasmatic neurons. Expression in neurons that were multipolar in shape, with a clear soma and a number of thin processes.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1G	Expression in cells arranged in inverted V-shaped array, with tip extending rostrally toward midline of ventral diencephalon where future chiam develops.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1H	Expression in chiasmatic neurons at central or midline domain of neuronal array. Expression in neurons that were multipolar in shape, with a clear soma and a number of thin processes.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2A	Expression in caudal region of diencephalon, in a V-shaped array of chiasmatic neurons 300m above the ventra pial surface.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2B	Expression at bifurcation at lateral region of neuronal array. Clear segregation of cells in lateral regions into a rostral and caudal division.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2C	Expression in chiasmatic neurons packed as a characteristic V-shaped configuration.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	2D	Expression in ventral diencephalon. Neuronal array straddled midline at level of chiasm and extended to initial segment of optic tract.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	2E	Expression in chiasmatic neurons extending rostrally to appose initial segment of optic tract.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	2F	Expression in lateral extensions from chiasmatic neurons consisting of both cells and processes. Processes form aense plexus at deep border of optic tract. Expression in some processes intruding into optic axon layer.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3A	Expression largely confined to two cellular domains and to deep border of optic tract.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3B	Restricted expression at initial segment of optic tract.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3C	Expression in chiasmatic neurons adjacent to optic tract.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3D	Expression in group of cells adjacent to midline and along deep border of optic tract.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3E	Expression localized in cells and processes along optic tract.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	3F	Expression adjacent to midline.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	3G	Expression localized in two distinct domains on each side of diencephalon and along ventricular regions.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	3H	Larger expression domain in rostral region next to midline.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4B , 4C	Expression in central domain of chiasmatic neurons, co-localized with Cd44, and in lateral regions of array.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4D	Co-localization with Cd44 expression in central domain of chiasmatic neurons, extending to chiasmatic midline. Predominate localization in lateral region of chiasmatic neurons apposing optic tract.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4E	Co-localization with Cd44 expression at central domain of array.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4F	At rostral level of chiasm, co-localization with Cd44 in chiasmatic neurons in central domain extending process into fiber layer at midline.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4G	At caudal level of chiasm, where axons enter optic tract, SSEA-1 expression predominates in chiasmatic neurons extending process forming deep plexus along deep border of tract.