Gene Expression Data
Assay Details
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Assay
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Reference:
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J:100603
Lin L, et al., Chiasmatic neurons in the ventral diencephalon of mouse embryos--changes in arrangement and heterogeneity in surface antigen expression. Brain Res Dev Brain Res. 2005 Aug 8;158(1-2):1-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7287754
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Gene symbol:
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Cd44
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Gene name:
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CD44 antigen
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Antibody:
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anti-CD44 (IM7)
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
4A/C:
embryonic day 14.5
(more  )
Note: Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
Specimen
4A/C:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4A
,
4C
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Restricted expression, co-localizing with SSEA-1, in central domain of chiasmatic neurons.
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Specimen
4D:
embryonic day 14.5
(more )
Note: Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4D
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Co-localization with SSEA-1 expression in central domain of chiasmatic neurons, extending to chiasmatic midline.
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Specimen
4E:
embryonic day 14.5
(more )
Note: Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
Specimen
4E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4E
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Co-localization with SSEA-1 expression at central domain of array.
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Specimen
4F:
embryonic day 14.5
(more )
Note: Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
Specimen
4F:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4F
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At rostral level of chiasm, co-localization with SSEA-1 expression in chiasmatic neurons in central domain extending process into fiber layer at midline.
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Specimen
4G:
embryonic day 14.5
(more )
Note: Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
Specimen
4G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Cd44; green - SSEA-1 (Fut4 assay MGI:7287753).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4G
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At caudal level of chiasm, where axons enter optic tract, lower Cd44 expression in chiasmatic neurons extending process forming plexus along deep border of tract.
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