Probe:	M2 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: nervous system	Strong		2A/B	Expression is in neural tissues and neural crest derivatives.
TS17: embryo endoderm	Present		2A/B
TS17: extraembryonic component	Present		2A/B
TS17: heart	Present		2A/B

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Strong		2C/D
TS21: forebrain	Strong		2C/D
TS21: hindbrain	Strong		2C/D
TS21: spinal cord	Strong		2C/D
TS21: thymus primordium	Strong		2C/D
TS21: sympathetic nervous system	Strong	Regionally restricted	2C/D	Expression is in the sympathetic plexus.
TS21: face	Strong	Regionally restricted	2C/D	Expression is in the facial mesectoderm.
TS21: heart	Present		2C/D
TS21: liver	Present		2C/D
TS21: midgut	Present		2C/D

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present		2E/F
TS25: face	Present		2E/F
TS25: gut	Present		2E/F
TS25: hindlimb phalanx	Present		2E/F
TS25: olfactory epithelium	Present		2E/F
TS25: tongue	Present		2E/F
TS25: heart	Weak		2E/F
TS25: thymus	Strong		2E/F

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cortical plate	neuron	Strong	Regionally restricted	4A/B	Expression is in migrating neurons and in neurons of the frontal cortical plate.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		4C/D

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		4E/F

Structure	Level	Pattern	Image	Note
TS24: lens	Present		4G/H
TS24: neural retina	Present		4G/H	Expression is predominantly in the retinal ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS24: alimentary system	Present	Regionally restricted	4I/J	Expression is in the submucosa.
TS24: enteric nervous system	Present		4I/J

Structure	Level	Pattern	Image	Note
TS24: heart atrium	Present		4K/L
TS24: heart ventricle	Present		4K/L

Structure	Level	Pattern	Image	Note
TS24: liver	Present		4M/N
TS24: thymus	Strong		4M/N

Structure	Level	Pattern	Image	Note
TS21: hindlimb mesenchyme	Strong		4O

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5A
TS28: anterior commissure	Weak		5A
TS28: corpus callosum	Weak		5A

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5C
TS28: corpus striatum	Present		5C
TS28: thalamic nucleus	Present		5C
TS28: dentate gyrus	Strong		5C	Expression was weaker in the glial regions.
TS28: hippocampus	Strong		5C	Expression was weaker in the glial regions.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5D
TS28: cerebellum	Present	Regionally restricted	5D	Expression was weaker in the glial regions.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Weak		5E/F
TS28: lateral ventricle ependyma	Weak		5E/F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spinal cord motor column	neuron	Strong		5G
TS28: spinal cord dorsal horn		Present		5G
TS28: spinal cord ventral horn		Present		5G
TS28: spinal cord		Present	Regionally restricted	5G	Expression was weaker in the glial regions.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: caudate-putamen	neuron	Strong		5H