Sp7 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:321550 Ting MC, et al., Embryonic requirements for Tcf12 in the development of the mouse coronal suture. Development. 2022 Jan 1;149(1):dev199575
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7284989
Gene symbol:	Sp7
Gene name:	Sp7 transcription factor 7

Antibody:	OSX Antibody (A-13): sc-22536-R
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Results

4D ©

Specimen 4D: embryonic day 14.5 (more

)
Note: Double labeled: magenta - Sp7; green - Grem1 (assay MGI:7284988).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present	Regionally restricted	4D	Expression is in osteolineage cells.
TS22: parietal bone primordium	Present	Regionally restricted	4D	Expression is in osteolineage cells.

4E ©

Specimen 4E: embryonic day 14.5; Tcf12^tm3Zhu/Tcf12^tm3Zhu (more

)
Note: Double labeled: magenta - Sp7; green - Grem1 (assay MGI:7284988).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present		4E
TS22: parietal bone primordium	Present		4E

4H ©

Specimen 4H: embryonic day 15.5 (more

)
Note: Triple labeled: magenta - Sp7; green - Twist1 (assay MGI:7284992); yellow - Tcf12 (assay MGI:7284995).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: frontal bone primordium	osteoblast	Present	Regionally restricted	4H	Expression is in osteoblasts.
TS23: parietal bone	osteoblast	Present	Regionally restricted	4H	Expression is in osteoblasts.

4I ©

Specimen 4I: embryonic day 15.5 (more

)
Note: Double labeled: magenta - Sp7; green - Twist1 (assay MGI:7284992).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: frontal bone primordium	osteoblast	Present	Regionally restricted	4I	Expression is in osteoblasts.
TS23: parietal bone	osteoblast	Present	Regionally restricted	4I	Expression is in osteoblasts.

4J ©

Specimen 4J: embryonic day 15.5 (more

)
Note: Double labeled: magenta - Sp7; yellow - Tcf12 (assay MGI:7284995).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: frontal bone primordium	osteoblast	Present	Regionally restricted	4J	Expression is in osteoblasts.
TS23: parietal bone	osteoblast	Present	Regionally restricted	4J	Expression is in osteoblasts.

4K ©

Specimen 4K: embryonic day 12.5 (more

)
Note: Double labeled: magenta - Sp7; green - Six2 (assay MGI:7284996).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: frontal bone primordium	osteoblast	Present	Regionally restricted	4K	Expression is in osteoblasts.
TS20: vault of skull	osteoblast	Present	Regionally restricted	4K	Expression is in osteoblasts.

4L ©

Specimen 4L: embryonic day 13.5 (more

)
Note: Double labeled: magenta - Sp7; green - Six2 (assay MGI:7284996).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: frontal bone primordium	osteoblast	Present	Regionally restricted	4L	Expression is in osteoblasts.
TS21: parietal bone primordium	osteoblast	Present	Regionally restricted	4L	Expression is in osteoblasts.

5G ©

Specimen 5G: embryonic day 13.5 (more

)
Note: Double labeled: magenta - Sp7; green - Efna2 (assay MGI:7284997).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: frontal bone primordium	osteoblast	Present	Regionally restricted	5G	Expression is in osteoblasts.
TS21: parietal bone primordium	osteoblast	Present	Regionally restricted	5G	Expression is in osteoblasts.

5H ©

Specimen 5H: embryonic day 13.5; Tcf12^tm3Zhu/Tcf12^tm3Zhu (more

)
Note: Double labeled: magenta - Sp7; green - Efna2 (assay MGI:7284997).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: frontal bone primordium	osteoblast	Present	Regionally restricted	5H	Expression is in osteoblasts.
TS21: parietal bone primordium	osteoblast	Present	Regionally restricted	5H	Expression is in osteoblasts.

S1A ©

Specimen S1A: embryonic day 14.5 (more

)
Note: Double labeled with BrdU (green).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present	Regionally restricted	S1A
TS22: parietal bone primordium	Present	Regionally restricted	S1A

S1B ©

Specimen S1B: embryonic day 14.5; Tcf12^tm3Zhu/Tcf12^tm3Zhu (more

)
Note: Double labeled with BrdU (green).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present	Regionally restricted	S1B	There is a 22% increase in the number of positive osteolineage cells along the bone fronts in this mutant compared to wild-type.
TS22: parietal bone primordium	Present	Regionally restricted	S1B	There is a 22% increase in the number of positive osteolineage cells along the bone fronts in this mutant compared to wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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