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Gene Expression Data
Assay Details
Assay
Reference: J:259302 Teratake Y, et al., Transcriptional repression of p27 is essential for murine embryonic development. Sci Rep. 2016 May 19;6:26244
Assay type: RT-PCR
MGI Accession ID: MGI:7284261
Gene symbol: Nes
Gene name: nestin
Probe: Nestin-pQ, Nestin-pR
Probe preparation: labelled with SYBR green
Assay notes: Relative expression was normalized to Gapdh and displayed relative to expression in wildtype.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
p27 HT Nczf WT E10.5 TS17: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ Cdkn1btm1Kin/Cdkn1b+Not Specified
p27 HT Nczf KO E10.5 TS17: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ Cdkn1btm1Kin/Cdkn1b+, Zfp949tm1Htno/Zfp949tm1HtnoNot Specified Conditional mutant.
p27 KO Nczf WT E10.5 TS17: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ Cdkn1btm1Kin/Cdkn1btm1KinNot Specified
p27 KO Nczf KO E10.5 TS17: embryo Strong (a) Not Specified; total RNA involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ Cdkn1btm1Kin/Cdkn1btm1Kin, Zfp949tm1Htno/Zfp949tm1HtnoNot Specified
Notes:
(a) Expression upregulated.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory