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Gene Expression Data
Assay Details
Assay
Reference: J:321576 Colombo S, et al., Stabilization of beta-catenin promotes melanocyte specification at the expense of the Schwann cell lineage. Development. 2022 Jan 15;149(2):dev194407
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7280727
Gene symbol: Pmel
Gene name: premelanosome protein
Antibody: Recombinant Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 3D/E: embryonic day 15.5; Ctnnb1tm1Mmt/Ctnnb1+ (more )
Note: Double labeled: red - Pmel; green - Tubb3 (assay MGI:7280737).
Structure Cell Type Level Pattern Image Note
TS23: autopod skin melanocyte Present 3E , 3D Some Pmel+ cells were in close proximity to Tubb3+ cells.
Specimen 3F/G: embryonic day 15.5; Ctnnb1tm1Mmt/Ctnnb1+, Tg(Tyr-cre)1Lru/0 (more )
Note: Conditional mutant. Double labeled: red - Pmel; green - Tubb3 (assay MGI:7280737).
Structure Cell Type Level Pattern Image Note
TS23: autopod skin melanocyte Present 3F , 3G Some Pmel+ cells were in close proximity to Tubb3+ cells. Ectopic melanocytes were seen.
Specimen 3H/I: postnatal day 1; Ctnnb1tm1Mmt/Ctnnb1+ (more )
Note: Double labeled: red - Pmel; green - Tubb3 (assay MGI:7280737).
Structure Cell Type Level Pattern Image Note
TS27: autopod skin melanocyte Present 3I , 3H Some Pmel+ cells were in close proximity to Tubb3+ cells.
Specimen 3J/K: postnatal day 1; Ctnnb1tm1Mmt/Ctnnb1+, Tg(Tyr-cre)1Lru/0 (more )
Note: Conditional mutant. Double labeled: red - Pmel; green - Tubb3 (assay MGI:7280737).
Structure Cell Type Level Pattern Image Note
TS27: autopod skin melanocyte Present 3K , 3J Some Pmel+ cells were in close proximity to Tubb3+ cells. Ectopic melanocytes were seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory