Gene Expression Data
Assay Details
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Assay
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Reference:
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J:181888
Ashe A, et al., Mutations in mouse Ift144 model the craniofacial, limb and rib defects in skeletal ciliopathies. Hum Mol Genet. 2012 Apr 15;21(8):1808-23
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7279185
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe212
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7A:
embryonic day 10.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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involves: FVB/NJ
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
7A
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Expression in posterior of limb.
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Specimen
7B:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19twto/Wdr19twto
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
7B
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Expression unaltered relative to controls.
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Specimen
7C:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19twto
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
7C
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Expression expanded anteriorly along distal periphery.
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Specimen
7D:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19dmhd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
7D
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Expression expanded anteriorly along distal periphery.
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Specimen
7M:
embryonic day 11.5
(more )
Specimen
7M:
(close )
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Genetic Background:
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involves: FVB/NJ
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7M
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Expression in posterior of limb.
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Specimen
7N:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19twto/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7N
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Expression unaltered relative to controls.
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Specimen
7O:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7O
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More discontinuous expanded domain of expression at E11.5.
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Specimen
S4A:
embryonic day 10.5
(more )
Specimen
S4A:
(close )
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Genetic Background:
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involves: FVB/NJ
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4A
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Expression in posterior of limb.
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Specimen
S4B:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19twto/Wdr19twto
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4B
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Expression unaltered relative to controls.
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Specimen
S4C:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19twto
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4C
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Expression not expanded anteriorly in hindlimb bud at E10.5.
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Specimen
S4D:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19dmhd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4D
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Expression not expanded anteriorly in hindlimb bud at E10.5.
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Analysis Tools