Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:181888
Ashe A, et al., Mutations in mouse Ift144 model the craniofacial, limb and rib defects in skeletal ciliopathies. Hum Mol Genet. 2012 Apr 15;21(8):1808-23
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7279185
|
Gene symbol:
|
Shh
|
Gene name:
|
sonic hedgehog
|
Probe:
|
Shh probe212
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
7A:
embryonic day 10.5
(more )
Specimen
7A:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
7A
|
Expression in posterior of limb.
|
Specimen
7B:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19twto/Wdr19twto
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
7B
|
Expression unaltered relative to controls.
|
Specimen
7C:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19dmhd/Wdr19twto
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
7C
|
Expression expanded anteriorly along distal periphery.
|
Specimen
7D:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19dmhd/Wdr19dmhd
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
7D
|
Expression expanded anteriorly along distal periphery.
|
Specimen
7M:
embryonic day 11.5
(more )
Specimen
7M:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
7M
|
Expression in posterior of limb.
|
Specimen
7N:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19twto/Wdr19twto
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
7N
|
Expression unaltered relative to controls.
|
Specimen
7O:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19dmhd/Wdr19twto
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
7O
|
More discontinuous expanded domain of expression at E11.5.
|
Specimen
S4A:
embryonic day 10.5
(more )
Specimen
S4A:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud |
Present |
Regionally restricted |
S4A
|
Expression in posterior of limb.
|
Specimen
S4B:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19twto/Wdr19twto
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud |
Present |
Regionally restricted |
S4B
|
Expression unaltered relative to controls.
|
Specimen
S4C:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19dmhd/Wdr19twto
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud |
Present |
Regionally restricted |
S4C
|
Expression not expanded anteriorly in hindlimb bud at E10.5.
|
Specimen
S4D:
(close )
Genetic Background:
|
involves: FVB/NJ
|
Mutant Allele(s):
|
Wdr19dmhd/Wdr19dmhd
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud |
Present |
Regionally restricted |
S4D
|
Expression not expanded anteriorly in hindlimb bud at E10.5.
|
|