Rest In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:322393 Kimura R, et al., Detection of REST expression in the testis using epitope-tag knock-in mice generated by genome editing. Dev Dyn. 2022 Mar;251(3):525-535
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7275283
Gene symbol:	Rest
Gene name:	RE1-silencing transcription factor

Reporter:	other (see notes)
Antibody:	anti-PA tag (clone NZ-1) (016-25861)
Detection system:	Secondary antibody coupled to Cy3
Assay notes:	Podoplanin, included in the allele construct, was used as the reporter in this assay.

Results

4_PA-tag RestPA/PA ©

Specimen 4 PA-tag RestPA/PA upper: embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	neural progenitor cell	Present	Regionally restricted	4_PA-tag RestPA/PA

4_PA-tag RestPA/PA (inset) ©

Specimen 4 PA-tag RestPA/PA upper inset: embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	neural progenitor cell	Present		4_PA-tag RestPA/PA (inset)

4_PA-tag/Pax6 RestPA/PA ©

Specimen 4 PA-tag RestPA/PA middle: embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Pax6 (assay MGI:7275289).

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present	Regionally restricted	4_PA-tag/Pax6 RestPA/PA

4_PA-tag/Pax6 RestPA/PA (inset) ©

Specimen 4 PA-tag RestPA/PA middle (inset): embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Pax6 (assay MGI:7275289).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	neural progenitor cell	Present		4_PA-tag/Pax6 RestPA/PA (inset)

4_PA-tag/Dcx RestPA/PA ©

Specimen 4 PA-tag RestPA/PA lower: embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Dcx (assay MGI:7275290).

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present	Regionally restricted	4_PA-tag/Dcx RestPA/PA

4_PA-tag/Dcx RestPA/PA (inset) ©

Specimen 4 PA-tag RestPA/PA lower (inset): embryonic day 13.5; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Dcx (assay MGI:7275290).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	neural progenitor cell	Present		4_PA-tag/Dcx RestPA/PA (inset)

5A PLZF PA-tag ©

5A PLZF PA-tag/Marker ©

Specimen 5A PA-tag upper: postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - PLZF (Zbtb16 assay MGI:7275291).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	5A PLZF PA-tag , 5A PLZF PA-tag/Marker

5A PLZF (inset) ©

Specimen 5A PA-tag upper (inset): postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - PLZF (Zbtb16 assay MGI:7275291).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		5A PLZF (inset)
TS28: testis	Present		5A PLZF (inset)

5A KIT PA-tag ©

5A KIT PA-tag/Marker ©

Specimen 5A PA-tag middle: postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Kit (assay MGI:7275292).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	5A KIT PA-tag , 5A KIT PA-tag/Marker

5A KIT (inset) ©

Specimen 5A PA-tag middle (inset): postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Kit (assay MGI:7275292).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		5A KIT (inset)
TS28: testis	Present		5A KIT (inset)

5A Sox9 PA-tag ©

5A Sox9 PA-tag/Marker ©

Specimen 5A PA-tag lower: postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Sox9 (assay MGI:7275293).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	5A Sox9 PA-tag , 5A Sox9 PA-tag/Marker

5A Sox9 (inset) ©

Specimen 5A PA-tag lower (insert): postnatal day 6; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Sox9 (assay MGI:7275293).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		5A Sox9 (inset)
TS28: testis	Present		5A Sox9 (inset)

6A PLZF PA-tag ©

Specimen 6A PA-tag upper: postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - PLZF (Zbtb16 assay MGI:7275291).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6A PLZF PA-tag , 6A PLZF PA-tag/Marker

Specimen 6A PA-tag upper (inset): postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - PLZF (Zbtb16 assay MGI:7275291).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		6A PLZF (inset)
TS28: testis	Present		6A PLZF (inset)

Specimen 6A PA-tag middle: postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Kit (assay MGI:7275292).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6A KIT PA-tag/Marker , 6A KIT PA-tag

Specimen 6A PA-tag middle (inset): postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Kit (assay MGI:7275292).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		6A KIT (inset)
TS28: testis	Present		6A KIT (inset)

Specimen 6A PA-tag lower: postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Sox9 (assay MGI:7275293).

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6A Sox9 PA-tag , 6A Sox9 PA-tag/Marker

Specimen 6A PA-tag lower (inset): postnatal adult; Rest^em1Nosu/Rest^em1Nosu (more

)
Note: Double labeled: red - Rest PA-tag; green - Sox9 (assay MGI:7275293).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		6A Sox9 (inset)
TS28: testis	Present		6A Sox9 (inset)

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