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Gene Expression Data
Assay Details
Assay
Reference: J:322393 Kimura R, et al., Detection of REST expression in the testis using epitope-tag knock-in mice generated by genome editing. Dev Dyn. 2022 Mar;251(3):525-535
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7275291
Gene symbol: Zbtb16
Gene name: zinc finger and BTB domain containing 16
Antibody: PLZF antibody (sc-22839)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5A PLZF: postnatal day 6; Restem1Nosu/Restem1Nosu (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: testis Present Regionally restricted 5A PLZF PA-tag/Marker , 5A PLZF Marker
Specimen 5A PLZF (inset): postnatal day 6; Restem1Nosu/Restem1Nosu (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5A PLZF (inset) Expression in undifferentiated spermatogonia.
TS28: testis Present Regionally restricted 5A PLZF (inset) Expression in undifferentiated spermatogonia.
Specimen 6A PLZF: postnatal adult; Restem1Nosu/Restem1Nosu (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: testis Present Regionally restricted 6A PLZF PA-tag/Marker , 6A PLZF Marker
Specimen 6A PLZF (inset): postnatal adult; Restem1Nosu/Restem1Nosu (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 6A PLZF (inset) Expression in undifferentiated spermatogonia.
TS28: testis Present Regionally restricted 6A PLZF (inset) Expression in undifferentiated spermatogonia.
Specimen 6C Rest+/+: postnatal adult (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: testis Present 6C Rest+/+
Specimen 6C RestPA/PA: postnatal adult (more )
Note: Double labeled: green - PLZF (Zbtb16); red - Rest PA-tag (assay MGI:7275283).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 6C RestPA/PA Expression in undifferentiated spermatogonia comparable to that of wild type.
TS28: testis Present Regionally restricted 6C RestPA/PA Expression in undifferentiated spermatogonia comparable to that of wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory