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Gene Expression Data
Assay Details
Assay
Reference: J:311212 Fantin A, et al., KIT Is Required for Fetal Liver Hematopoiesis. Front Cell Dev Biol. 2021;9:648630
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7263439
Gene symbol: Cdh5
Gene name: cadherin 5
Antibody: anti-CD144 (555289)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-VE-cadherin MGI:6723790 may have been used in this assay.
Results
Specimen 1B: embryonic day 9.5 (more )
Note: Multi-labeled: green - Cdh5; red - Kit (assay MGI:7263441); white - Kdr (assay MGI:7263442); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS15: yolk sac endothelial cell Present Regionally restricted 1B left Expression in cytoplasm of endothelial cells (ECs).
TS15: yolk sac yolk sac hematopoietic stem cell Present Regionally restricted 1B left Expression in cytoplasm of some erythromyeloid progenitors (EMPs).
Specimen 1B inset: embryonic day 9.5 (more )
Note: Multi-labeled: green - Cdh5; red - Kit (assay MGI:7263441); white - Kdr (assay MGI:7263442); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS15: yolk sac yolk sac hematopoietic stem cell Present Regionally restricted 1B center left , 1B right Expression in Kit-expressing EMPs budding from hemogenic endothelium.
Specimen 1C: embryonic day 9.5 (more )
Note: Double labeled: green - Cdh5; red - Kit (assay MGI:7263441).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac Kit-positive erythroid progenitor cell Present Regionally restricted 1C left Greater intracellular expression in Kit-expressing EMPs budding than in neighboring ECs.
Specimen 1C lateral views: embryonic day 9.5 (more )
Note: Double labeled: green - Cdh5; red - Kit (assay MGI:7263441).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac Kit-positive erythroid progenitor cell Present Regionally restricted 1C right upper , 1C center lower , 1C right lower , 1C center upper No expression in EMPs released into vessel lumen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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