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Gene Expression Data
Assay Details
Assay
Reference: J:311212 Fantin A, et al., KIT Is Required for Fetal Liver Hematopoiesis. Front Cell Dev Biol. 2021;9:648630
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7263441
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-KIT (553353)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-KIT MGI:7263435 may have been used in this assay.
Results
Specimen 1B: embryonic day 9.5 (more )
Note: Multi-labeled: red - Kit; green - Cdh5 (assay MGI:7263439); white - Kdr (assay MGI:7263442); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS15: yolk sac endothelial cell Present Clusters 1B left Expression in small clusters of cells within endothelium .
Specimen 1B inset: embryonic day 9.5 (more )
Note: Multi-labeled: red - Kit; green - Cdh5 (assay MGI:7263439); white - Kdr (assay MGI:7263442); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS15: yolk sac yolk sac hematopoietic stem cell Present Regionally restricted 1B center left , 1B center right Expression in cells within endothelium that appeared rounder and smaller than neighboring endothelial cells (ECs).
TS15: yolk sac endothelial cell Present Regionally restricted 1B center left , 1B center right No expression in non-hemogenic endothelial cells.
Specimen 1C: embryonic day 9.5 (more )
Note: Double labeled: red - Kit; green - Cdh5 (assay MGI:7263439).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac yolk sac hematopoietic stem cell Present Regionally restricted 1C left Expression in erythromyeloid progenitors (EMPs) budding into vascular lumen.
Specimen 1C lateral views: embryonic day 9.5 (more )
Note: Double labeled: red - Kit; green - Cdh5 (assay MGI:7263439).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac yolk sac hematopoietic stem cell Present Regionally restricted 1C center lower , 1C center upper Expression in erythromyeloid progenitors (EMPs) budding into vascular lumen.
Specimen 4F: embryonic day 12.5 (more )
Note: Double labeled: red - Kit; green - TER119 (Ly76 assay MGI:7263444).
Structure Cell Type Level Pattern Image Note
TS20: liver fetal liver hematopoietic progenitor cell Present 4F Expression in large and round cells likely to represent hematopoietic progenitors.
TS20: liver proerythroblast Present Regionally restricted 4F Expression in only a few Ter119+ proerythroblasts.
TS20: liver erythroblast Absent 4F No expression in Ter119+ erythroblasts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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