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Gene Expression Data
Assay Details
Assay
Reference: J:320108 Chau TCY, et al., Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice. Dev Dyn. 2022 Feb;251(2):336-349
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7262865
Gene symbol: Golim4
Gene name: golgi integral membrane protein 4
Antibody: Anti-GOLPH4 antibody (ab28049)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2G/G': embryonic day 13.5 (more )
Note: Also stained for Prox1 (assay MGI:7262864).
Structure Level Pattern Image Note
TS21: back skin Present Regionally restricted 2G' , 2G Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. The Golgi body consistently aligned in the direction of migration in wild type sprouts.
TS21: dermis Present Regionally restricted 2G' , 2G Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. The Golgi body consistently aligned in the direction of migration in wild type sprouts.
TS21: lymph sac Present Regionally restricted 2G' , 2G Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. The Golgi body consistently aligned in the direction of migration in wild type sprouts.

Specimen 2H/H': embryonic day 13.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Also stained for Prox1 (assay MGI:7262864).
Structure Level Pattern Image Note
TS21: back skin Present Regionally restricted 2H , 2H' Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. Alignment was more randomized and less directional in mutant mice.
TS21: dermis Present Regionally restricted 2H , 2H' Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. Alignment was more randomized and less directional in mutant mice.
TS21: lymph sac Present Regionally restricted 2H , 2H' Expressed in the Golgi body in the stained subcutaneous lymphatic sprouts. Alignment was more randomized and less directional in mutant mice.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory