Probe:	Dbx2 cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Weak	Regionally restricted	1A E10	Expressed throughout the limb bud mesenchyme, with the exception of mesodermal cells underlying the distal-most limb ectoderm.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1A E11	Expression was confined to a small and posterior moon-shaped cellular domain, and in the anterior portion of the autopod.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Widespread	1A E12
TS20: forelimb interdigital region mesenchyme	Present	Widespread	1A E12

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present		1A E13
TS21: hand joint	Present		1A E13

Structure	Level	Pattern	Image	Note
TS22: hand joint	Present		1A E14

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present		5D left
TS21: hand joint	Present		5D left

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present		5D right	Reduced expression compared with the wild-type.
TS21: hand joint	Present		5D right	Reduced expression compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present		6B-1
TS20: forelimb interdigital region mesenchyme	Present		6B-1

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Weak		6B-2	Strongly decreased expression compared with the wild-type. Expressed in the distal portion of the central digits only.
TS20: forelimb interdigital region mesenchyme	Weak		6B-2	Strongly decreased expression compared with the wild-type. Expressed in the distal portion of the central digits only.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present		6B-3	Only a weak reduction in expression compared with the wild-type, with the exception of the posterior-most portion of the autopod, where expression was severely reduced.
TS20: forelimb interdigital region mesenchyme	Present		6B-3	Only a weak reduction in expression compared with the wild-type, with the exception of the posterior-most portion of the autopod, where expression was severely reduced.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Absent		6B-4
TS20: forelimb interdigital region mesenchyme	Absent		6B-4

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Absent		6B-5
TS20: forelimb interdigital region mesenchyme	Absent		6B-5

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Trace		6B-6	Expression was almost completely abrogated. A faint and spatially ill-defined signal was seen.

Structure	Level	Pattern	Image	Note
TS18: central nervous system	Present		8C E11 Dbx2
TS18: limb bud	Present		8C E11 Dbx2

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present		8C E13 Dbx2
TS21: hand joint	Present		8C E13 Dbx2