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Gene Expression Data
Assay Details
Assay
Reference: J:248980 Guo H, et al., Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017 Oct 1;430(1):214-223
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7258078
Gene symbol: Pdpn
Gene name: podoplanin
Antibody: Anti-Podoplanin (clone 8.1.1)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A Alveolar WT Pdpn: embryonic day 18.5 (more )
Note: Triple labeled: red - Pdpn; green - Sftpc (assay MGI:7258076); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus type I pneumocyte Present 2A Alveolar WT +DAPI , 2A Alveolar WT Pdpn Expression in alveolar epithelial type 1 (AT1) cells in distal saccules.
Specimen 2A Alveolar TNRC6c-/- Pdpn: embryonic day 18.5; Tnrc6cGt(IST11346C2)Tigm/Tnrc6cGt(IST11346C2)Tigm (more )
Note: Triple labeled: red - Pdpn; green - Sftpc (assay MGI:7258076); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus type I pneumocyte Present 2A Alveolar TNRC6c-/- +DAPI , 2A Alveolar TNRC6c-/- Pdpn Expression present and properly localized within mutant lungs, in a similar to that of wild type.
Specimen 2B Alveolar WT Pdpn: postnatal day 0 (more )
Note: Triple labeled: red - Pdpn; green - Sftpc (assay MGI:7258076); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: lung alveolus type I pneumocyte Present 2B Alveolar WT Pdpn , 2B Alveolar WT +DAPI Expression in alveolar epithelial type 1 (AT1) cells in distal saccules.
TS27: lung alveolus Present 2B Alveolar WT +DAPI , 2B Alveolar WT pSftpc Expression present and properly localized within mutant lungs, in a similar to that of wild type.
Specimen 2B Alveolar TNRC6c-/- Pdpn: postnatal day 0; Tnrc6cGt(IST11346C2)Tigm/Tnrc6cGt(IST11346C2)Tigm (more )
Note: Triple labeled: red - Pdpn; green - Sftpc (assay MGI:7258076); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: lung alveolus type II pneumocyte Present 2B Alveolar TNRC6c-/- +DAPI , 2B Alveolar TNRC6c-/- Pdpn Expression present and properly localized within mutant lungs, in a similar to that of wild type.
Specimen 4A WT Pdpn: embryonic day 18.5 (more )
Note: Triple labeled: green - Pdpn; red - Pecam1 (assay MGI:7258083) and Plvap (MECA32; assay MGI:7258085); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus epithelial cell of alveolus of lung Present Regionally restricted 4A WT (left) , 4A WT (right) , 4A WT (middle) Expression on epithelial surface in wild type and mutant distal lung. Tight apposition of epithelial and endothelial cells.
Specimen 4A TNRC6c-/- Pdpn: embryonic day 18.5; Tnrc6cGt(IST11346C2)Tigm/Tnrc6cGt(IST11346C2)Tigm (more )
Note: Triple labeled: green - Pdpn; red - Pecam1 (assay MGI:7258083) and Plvap (MECA32; assay MGI:7258085); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus epithelial cell of alveolus of lung Present Regionally restricted 4A TNRC6c-/- (left) , 4A TNRC6c-/- (middle) , 4A TNRC6c-/- (right) Lack of close physical association between endothelial and epithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory