Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:321168
Wang H, et al., GCNF-dependent activation of cyclin D1 expression via repression of Mir302a during ESC differentiation. Stem Cells. 2014 Jun;32(6):1527-37
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7254857
|
|
Gene symbol:
|
Ccnd1
|
|
Gene name:
|
cyclin D1
|
|
Antibody:
|
anti-cyclin D1
|
|
Detection system:
|
Secondary antibody coupled to Texas Red
|
|
|
Results
|
Specimen
2D wt:
embryonic day 8.5
(more  )
Specimen
2D wt:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Widespread |
2D wt merge
,
2D wt Cyclin D1
|
Expression in most tissues.
|
| TS13: neural ectoderm |
Strong |
|
2D wt merge
,
2D wt Cyclin D1
|
High levels of expression in neural epithelium.
|
Specimen
2D GCNF-/-:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Nr6a1tm1Coo/Nr6a1tm1Coo
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Absent |
|
2D GCNF-/- merge
,
2D GCNF-/- Cyclin D1
|
|
|
Analysis Tools
