Focad Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:310838 Liu D, et al., PRICKLE1 x FOCAD Interaction Revealed by Genome-Wide vQTL Analysis of Human Facial Traits. Front Genet. 2021;12:674642
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6887446
Gene symbol:	Focad
Gene name:	focadhesin

Antibody:	anti-Focadhesin (HPA055015)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

7A +/+ ©

7A +/+ (red) ©

Specimen 7A +/+: embryonic day 12.5 (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Ubiquitous	7A +/+ (red) , 7A +/+	Ubiquitous expression in cranium base mesenchyme, colocalized with Prickle1.

7B +/+ ©

7B +/+ (red) ©

Specimen 7B +/+: embryonic day 15.5 (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cranium	chondrocyte	Present	Regionally restricted	7B +/+ (red) , 7B +/+	Colocalized with Prickle1 at cleavage furrow.

7C Bj/Bj ©

Specimen 7C Bj/Bj: embryonic day 12.5; Prickle1^b2b019Clo/Prickle1^b2b019Clo (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Punctate	7C Bj/Bj (red) , 7C Bj/Bj	Expression cranium base mesenchyme, colocalized with Prickle1 in puncta at cell periphery.

7D Bj/Bj ©

Specimen 7D Bj/Bj: embryonic day 15.5; Prickle1^b2b019Clo/Prickle1^b2b019Clo (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cranium	chondrocyte	Present	Regionally restricted	7D Bj/Bj , 7D Bj/Bj (red)	Colocalized with Prickle1 in numerous puncta and some cleavage furrows.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24