Focad Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:310838 Liu D, et al., PRICKLE1 x FOCAD Interaction Revealed by Genome-Wide vQTL Analysis of Human Facial Traits. Front Genet. 2021;12:674642
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6887446
Gene symbol:	Focad
Gene name:	focadhesin

Antibody:	anti-Focadhesin (HPA055015)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

7A +/+ ©

7A +/+ (red) ©

Specimen 7A +/+: embryonic day 12.5 (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Ubiquitous	7A +/+ (red) , 7A +/+	Ubiquitous expression in cranium base mesenchyme, colocalized with Prickle1.

7B +/+ ©

7B +/+ (red) ©

Specimen 7B +/+: embryonic day 15.5 (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cranium	chondrocyte	Present	Regionally restricted	7B +/+ (red) , 7B +/+	Colocalized with Prickle1 at cleavage furrow.

7C Bj/Bj ©

Specimen 7C Bj/Bj: embryonic day 12.5; Prickle1^b2b019Clo/Prickle1^b2b019Clo (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Punctate	7C Bj/Bj (red) , 7C Bj/Bj	Expression cranium base mesenchyme, colocalized with Prickle1 in puncta at cell periphery.

7D Bj/Bj ©

Specimen 7D Bj/Bj: embryonic day 15.5; Prickle1^b2b019Clo/Prickle1^b2b019Clo (more

)
Note: Triple labeled: red - Focad; green - Prickle1 (assay MGI:6887447); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cranium	chondrocyte	Present	Regionally restricted	7D Bj/Bj , 7D Bj/Bj (red)	Colocalized with Prickle1 in numerous puncta and some cleavage furrows.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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