Gene Expression Data
Assay Details
Assay
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Reference:
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J:321365
Knoedler JR, et al., A functional cellular framework for sex and estrous cycle-dependent gene expression and behavior. Cell. 2022 Feb 17;185(4):654-671.e22
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6886064
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Gene symbol:
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Esr1
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Gene name:
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estrogen receptor 1 (alpha)
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Probe:
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Esr1 probe2
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Probe preparation:
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Antisense labelled with Alexa Fluor
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Visualized with:
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Fluorescence
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Results
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Specimen
3C - Nrip1:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Nrip1 (assay MGI:6886031)
Specimen
3C - Nrip1:
(close )
Genetic Background:
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C57BL/6J
|
Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Esr1; green - Nrip1 (assay MGI:6886031)
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: principal nucleus |
neuron |
Present |
|
3C Nrip1 - Merge
,
3C Nrip1 - Esr1
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Expression is seen in the principal component of the bed nucleus of the stria terminalis (BNSTpr).
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Specimen
3C - Pgr15l:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Pgr15l (assay MGI:6886053)
Specimen
3C - Pgr15l:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: red - Esr1; green - Pgr15l (assay MGI:6886053)
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: principal nucleus |
neuron |
Present |
|
3C Pgr15l - Esr1
,
3C Pgr15l - Merge
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Expression is seen in the principal component of the bed nucleus of the stria terminalis (BNSTpr).
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Specimen
3C - Col25a1:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Col25a1 (assay MGI:6886073)
Specimen
3C - Col25a1:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Esr1; green - Col25a1 (assay MGI:6886073)
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial amygdaloid nucleus |
neuron |
Present |
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3C Col25a1 - Esr1
,
3C Col25a1 - Merge
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Expression is seen in the medial amygdala (MeA).
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Specimen
3C - Cckar:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Cckar (assay MGI:6886052)
Specimen
3C - Cckar:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Esr1; green - Cckar (assay MGI:6886052)
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial amygdaloid nucleus |
neuron |
Present |
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3C Cckar - Esr1
,
3C Cckar - Merge
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Expression is seen in the medial amygdala (MeA).
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Specimen
3C - Crim1:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Crim1 (assay MGI:6886028)
Specimen
3C - Crim1:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Esr1; green - Crim1 (assay MGI:6886028)
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: preoptic area |
neuron |
Present |
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3C Crim1 - Esr1
,
3C Crim1 - Merge
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Expression is seen in the preoptic hypothalamus (POA).
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Specimen
3C - Pappa:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Pappa (assay MGI:6886027)
Specimen
3C - Pappa:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Esr1; green - Pappa (assay MGI:6886027)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: preoptic area |
neuron |
Present |
|
3C Pappa - Esr1
,
3C Pappa - Merge
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Expression is seen in the preoptic hypothalamus (POA).
|
Specimen
3C - PR:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Pgr (assay MGI:6886072)
Specimen
3C - PR:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Esr1; green - Pgr (assay MGI:6886072)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: ventromedial hypothalamic nucleus |
neuron |
Present |
Regionally restricted |
3C PR - Merge
,
3C PR - Esr1
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Expression is seen in the ventromedial hypothalamus ventrolateralis (VMHvl).
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Specimen
3C - Abtb2:
postnatal adult
(more )
Note: Double labeled: red - Esr1; green - Abtb2 (assay MGI:6886048)
Specimen
3C - Abtb2:
(close )
Genetic Background:
|
C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Esr1; green - Abtb2 (assay MGI:6886048)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: ventromedial hypothalamic nucleus |
neuron |
Present |
Regionally restricted |
3C Abtb2 - Merge
,
3C Abtb2 - Esr1
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Expression is seen in the ventromedial hypothalamus ventrolateralis (VMHvl).
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Specimen
6B:
postnatal adult
(more )
Note: Double labeled: green - Esr1; red- Tac1 (assay MGI:6886029)
Specimen
6B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Esr1; red- Tac1 (assay MGI:6886029)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: principal nucleus |
neuron |
Present |
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6B Merge
,
6B Esr1
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Expression is seen in the principal component of the bed nucleus of the stria terminalis (BNSTpr).
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Specimen
7C:
postnatal adult
(more )
Note: Double labeled: green - Esr1; red - Cckar (assay MGI:6886052)
Specimen
7C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Esr1; red - Cckar (assay MGI:6886052)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: ventromedial hypothalamic nucleus |
Present |
Regionally restricted |
7C Merge
,
7C Esr1
|
Expression is seen in the ventromedial hypothalamus ventrolateralis (VMHvl).
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