Gene Expression Data
Assay Details
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Assay
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Reference:
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J:306594
De Maria A, et al., Expression of potassium-dependent sodium-calcium exchanger in the murine lens. Exp Eye Res. 2018 Feb;167:18-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6865730
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Gene symbol:
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Slc24a4
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Gene name:
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solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
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Probe:
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Mm-Slc24a4 (313991)
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Probe preparation:
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Antisense labelled with alkaline phosphatase
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Visualized with:
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Chromogenic
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Results
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Specimen
E12 not shown:
embryonic day 12.0
(more  )
Specimen
E12 not shown:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2A:
embryonic day 16.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: lens fiber |
nucleated secondary lens fiber |
Strong |
Regionally restricted |
2A
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Abundant expression in nucleated secondary lens fiber cells.
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| TS24: lens fiber |
non-nucleated secondary lens fiber |
Weak |
Regionally restricted |
2A
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Low expression in central fiber cells undergoing undergoing nuclear degradation.
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| TS24: lens epithelium |
lens epithelial cell |
Trace |
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2A
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Almost undetectable expression in epithelial cells.
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Specimen
2B:
postnatal day 30
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2B
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Abundant expression in lens fiber cells, restricted to fibers in outer lens cortex. No expression in inner cortical region bordering the organelle-free zone (OFZ)
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| TS28: lens |
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Present |
Regionally restricted |
2B
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No expression in cells of organelle-free zone (OFZ) itself.
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| TS28: lens epithelium |
lens epithelial cell |
Trace |
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2B
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Almost undertectable expression in epithelial cells.
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Analysis Tools