Antibody:	anti-Ric8
Detection system:	Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Diffuse	3C , 3A	When chromatin starts to condense as a partial rim around the nucleolus, expression remained diffusely localized across germinal vesicle.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Regionally restricted	3c , 3a	Expression partially co-localized with NUMA (Numa1).

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Regionally restricted	3F , 3D	When nuclear lamina and nucleolus start to disappear, expression remain in vicinity of chromosomes.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Spotted	3d , 3f	Discrete spots of expression in close vicinity of chromosomes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Spotted	3I , 3G	Expression accumulated in spots distributed around condensed chromatin in germinal vesicle, with some foci attached to nucleolus.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Regionally restricted	3i , 3g	No co-localization with LGN (Gpsm2).

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Spotted	3J , 3L	Expression accumulated in spots distributed around condensed chromatin in germinal vesicle, with some foci attached to nucleolus.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Regionally restricted	3l , 3j	No significant overlap with Galphai1/2 (Gnai1, Gnai2).

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	4A , 4C	With organization of chromosomes to the metaphase plate, expressioon distributed along metaphase spindle. High extent of co-localization with LGN (Gpsm2) at meiotic spindle apparatus.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	4D , 4F	During chromosome separation, expression remained in vicinity of chromosomes, localized with meiotic spindle. Expression condensed more at plus-end of meiotic spindle.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Regionally restricted	4G , 4I	During second arrest, expression distributed to metaphase spindle and diffusely in cytoplasm. Extent of colocalization with LGN (Gpsm2) similar to MI oocytes.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	5C , 5A	When chromosomes segregate, expressioWhen chromosomes segregate, expression translocated to spindle midzone. Expression also detected uniformly in cytoplasm of oocyte, and also in some extent in cell cortex. n translocated to spindle midzone. Expression also detected uniformly in cytoplasm of oocyte, and also in some extent in cell cortex. When chromosomes segregate, expression translocated to spindle midzone. Expression also detected uniformly in cytoplasm of oocyte, and also in some extent in cell cortex.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	5F , 5D	Abundant expression in meiotic spindle. Expression detected uniformly in cytoplasm of oocyte, and also in some extent in cell cortex.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	6A , 6C	Expression localized in pronucleus as discreted spots around nucleoli.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Strong	Regionally restricted	6a , 6c	Strong expression in nucleolus precursor bodies (NPBs).

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	6F , 6D	Expression localized in pronucleus
TS1: second polar body	Strong	Regionally restricted	6D , 6F	High level of expression in spindle of second polar body, colocalized with LGN (Gpsm2) in middle of spindle, but not at plus or minus ends.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	6d , 6f	Expression in pronucleus as discreted spots around nucleoli.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	6G , 6I	Expression in cell cortex.
TS1: second polar body	Present		6G , 6I

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	6i , 6g	Expression in cell cortex.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	7C , 7A	Expression shifted to metaphase plate in close vicinity of chromosomes and microtubules. Co-localized with LGN (Gpsm2) and Galphai1/2 (Gnai1, Gnai2) in mitotic spndle and cell cortex. In cell cortex, typcially co-localized with Galphai1/2 (Gnai1, Gnai2).

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	7D , 7F	Expression localized in apical region of cell cortex, with specific exclusion from region of cell-cell contact. In cell cortex, typcially co-localized with Galphai1/2 (Gnai1, Gnai2).

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	7d , 7f	In apical cortex region of blastomeres, less overlap with Galphai1/2 (Gnai1, Gnai2).

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	7I , 7G	Expression localized in apical region of cell cortex, with specific exclusion from region of cell-cell contact. In cell cortex, typcially co-localized with Galphai1/2 (Gnai1, Gnai2) and LGN (Gpsm2).

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	7i , 7g	In apical cortex region of blastomeres, extensive ovarlap with LGN (Gpsm2).

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	7L , 7J	Expression localized in apical region of cell cortex, with specific exclusion from region of cell-cell contact. No overlap with NUMA (Numa1) in nucleus.