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Gene Expression Data
Assay Details
Assay
Reference: J:237701 Saare M, et al., Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One. 2015;10(6):e0129131
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6864142
Gene symbol: Gnai1
Gene name: G protein subunit alpha i1
Antibody: anti-Galphai1/2
Detection system: Alexa Fluor
Results
Specimen 3K/L: postnatal adult (more )
Note: germinal vesicle stage. Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Present Uniform 3L , 3K Uniform distribution of expression in cytoplasm of oocyte.
Specimen 3k/l: postnatal adult (more )
Note: Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Present Regionally restricted 3l , 3k No significant overlap with RIC8 (Ric8a).
Specimen 5E/F: postnatal adult (more )
Note: anaphase/telophase of meiosis I. Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 5E , 5F Expression remained in cortex area Expression only weakly detectable in meiotic spindle.
Specimen not shown: postnatal adult (more )
Note: metaphase of meiosis II.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted Expression remained in cortex area.
Specimen 6H/I: embryonic day 0.5 (more )
Note: pronuclear stage. Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 6I , 6H Expression in remnants of spindle and cell cortex.
TS1: second polar body Present 6I , 6H
Specimen 6h/i: embryonic day 0.5 (more )
Note: Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 6h , 6i Expression in remnants of spindle and cell cortex.
Specimen 7B/C: embryonic day 0.5 (more )
Note: metaphase I of first mitosis. Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 7B , 7C Co-localized with RIC8 (Ric8a) and LGN (Gpsm2) in mitotic spndle and cell cortex.
Specimen 7E/F: embryonic day 1.0 (more )
Note: Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 7E , 7F In cell cortex, typcially co-localized with RIC8 (Ric8a) and LGN (Gpsm2). Strong expression in region between blastomeres.
Specimen 7e/f: embryonic day 1.0 (more )
Note: Triple labeled: red - Gnai1 and Gnai2; green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 7f , 7e In apical cortex region of blastomeres, less overlap with RIC8 (Ric8a).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory