Scgb1a1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6863929
Gene symbol:	Scgb1a1
Gene name:	secretoglobin, family 1A, member 1

Antibody:	anti-Scgb1a1
Detection system:	Secondary antibody

Results

1F center Lineage Marker ©

1F center merge ©

Specimen 1F - Scgb1a1: postnatal adult; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Sfta3-ps reporter (assay MGI:6863897).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		1F center merge , 1F center Lineage Marker	Expression is seen in secretory cells.

1H center Lineage Marker ©

1H center merge ©

Specimen 1H - Scgb1a1: postnatal adult (more

)
Note: Double labeled: green - Scgb1a1; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		1H center Lineage Marker , 1H center merge	Expression is seen in secretory cells.

3B upper Wild-type ©

Specimen 3B - Wild-type: embryonic day 18.5 (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B upper Wild-type	Expression is seen in secretory cells.

3B upper NANCI^RFP/+ ©

Specimen 3B - NANCI^{creERT2-RFP/+}: embryonic day 18.5; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B upper NANCI^RFP/+	Expression is seen in secretory cells; their numbers are comparable to those seen in wild type.

3B upper Nkx2.1^GFP/+ ©

Specimen 3B - Nkx2.1^GFP/+: embryonic day 18.5; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B upper Nkx2.1^GFP/+	Expression is seen in secretory cells; their numbers are significantly reduced compared to those seen in wild type.

3B upper NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 3B - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: embryonic day 18.5; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B upper NANCI^RFP/+; Nkx2.1^GFP/+	Expression is seen in secretory cells; their numbers are significantly reduced compared to those seen in wild type.

3E upper Wild-type ©

Specimen 3E - Wild-type: postnatal day 4 (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E upper Wild-type	Expression is seen in secretory cells.

3E upper NANCI^RFP/+ ©

Specimen 3E - NANCI^{creERT2-RFP/+}: postnatal day 4; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E upper NANCI^RFP/+	Expression is seen in secretory cells; their numbers are comparable to those seen in wild type.

3E upper Nkx2.1^GFP/+ ©

Specimen 3E - Nkx2.1^GFP/+: postnatal day 4; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E upper Nkx2.1^GFP/+	Expression is seen in secretory cells; unlike earlier in development, their numbers are comparable to those seen in wild type.

3E upper NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 3E - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal day 4; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E upper NANCI^RFP/+; Nkx2.1^GFP/+	Expression is seen in secretory cells; their numbers are significantly reduced compared to those seen in wild type.

5B ©

Specimen 5B: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Sox2 (assay MGI:6863946).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	5B	Expression is seen in the bronchiolized cells in the distal lung, suggesting they are primarily secretory cells.

5F ©

Specimen 5F: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Sftpc (assay MGI:6863924).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	5F	Expression is seen in the bronchiolized cells in the distal lung, suggesting they are primarily secretory cells.

Specimen 6C - Wild-type: postnatal month 6 (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		6C top Wild-type

Specimen 6C - NANCI^{creERT2-RFP/+}: postnatal month 6; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		6C top NANCI^RFP/+

Specimen 6C - Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		6C top Nkx2.1^GFP/+

Specimen 6C - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		6C top NANCI^RFP/+; Nkx2.1^GFP/+	Secretory cell expression is reduced in the mutant.

6D ©

Specimen 6D: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6863930).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		6D	Secretory cell expression is seen in the distal bronchiolized epithelium.

S8I ©

Specimen S8I: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Scgb1a1; red - Mki67 (assay MGI:6863973).

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Single cells	S8I	In response to the ongoing injury in the mutant, the secretory cells are proliferating. This is quantified in Fig. S8K.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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