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Gene Expression Data
Assay Details
Assay
Reference: J:308824 de Guia RM, et al., Ablation of Nampt in AgRP neurons leads to neurodegeneration and impairs fasting- and ghrelin-mediated food intake. FASEB J. 2021 May;35(5):e21450
Assay type: RT-PCR
MGI Accession ID: MGI:6850151
Gene symbol: Pomc
Gene name: pro-opiomelanocortin-alpha
Probe: Pomc-pM, Pomc-pN
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR was used to obtain these results. Gene expression was normalized to either 18S rRNA content or to total cDNA content.
Results Image: 4C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
ARC WT P w 21 TS28: arcuate nucleus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteMale
ARC ARNKO P w 21 TS28: arcuate nucleus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Male Conditional mutant.
HPC WT P w 21 TS28: hippocampus Weak 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteMale
HPC ARNKO P w 21 TS28: hippocampus Weak 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Male Conditional mutant.
Cortex WT P w 21 TS28: cerebral cortex Weak 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteMale
Cortex ARNKO P w 21 TS28: cerebral cortex Weak 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Male Conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory